High density growth of T7 expression strains with auto-induction option

Studier, F. William

High density growth of T7 expression strains with auto-induction option

Patent · Tue Mar 19 04:00:00 EDT 2013

OSTI ID:1083033

Studier, F. William

A method for promoting and suppressing auto-induction of transcription of a cloned gene 1 of bacteriophage T7 in cultures of bacterial cells grown batchwise is disclosed. The transcription is under the control of a promoter whose activity can be induced by an exogenous inducer whose ability to induce said promoter is dependent on the metabolic state of said bacterial cells.

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Research Organization:: BNL (Brookhaven National Laboratory (BNL), Upton, NY (United States))

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-98CH10886

Assignee:: Brookhaven Science Associates, LLC (Upton, NY)

Patent Number(s):: 8,399,217

Application Number:: 11/741,282

OSTI ID:: 1083033

Country of Publication:: United States

Language:: English

References (19)

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