Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Sommer, Gregory J; Hatch, Anson V; Singh, Anup K; Wang, Ying-Chih

Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Patent · Tue Oct 28 04:00:00 EDT 2014

OSTI ID:1162116

Sommer, Gregory J; Hatch, Anson V; Singh, Anup K; Wang, Ying-Chih

Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.

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Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia Corporation (Albuquerque, NM)

Patent Number(s):: 8,871,496

Application Number:: 12/544,982

OSTI ID:: 1162116

Country of Publication:: United States

Language:: English

References (44)

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