Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.

Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

Patent · Tue Nov 17 04:00:00 EST 2015

OSTI ID:1226547

Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.

Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.

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Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia Corporation (Albuquerque, NM)

Patent Number(s):: 9,186,668

Application Number:: 12/891,956

OSTI ID:: 1226547

Country of Publication:: United States

Language:: English

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