CBH1 homologs and varian CBH1 cellulase

Goedegebuur, Frits; Gualfetti, Peter; Mitchinson, Colin; Neefe, Paulien

CBH1 homologs and varian CBH1 cellulase

Patent · Tue Jul 01 04:00:00 EDT 2014

OSTI ID:1136635

Goedegebuur, Frits; Gualfetti, Peter; Mitchinson, Colin; Neefe, Paulien

Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.

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Research Organization:: Danisco US Inc., Palo Alto, CA (USA)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC36-99GO10337

Assignee:: Danisco US Inc. (Palo Alto, CA)

Patent Number(s):: 8,765,440

Application Number:: 13/072,516

OSTI ID:: 1136635

Country of Publication:: United States

Language:: English

References (97)

Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli Spilliaert, Remi; Hreggvidsson, Gudmundur Oli; Kristjansson, Jakob Kristjan European Journal of Biochemistry, Vol. 224, Issue 3, p. 923-930 https://doi.org/10.1111/j.1432-1033.1994.00923.x	journal	September 1994
A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I Ståhlberg, Jerry; Johansson, Gunnar; Pettersson, Göran Nature Biotechnology, Vol. 9, Issue 3, p. 286-290 https://doi.org/10.1038/nbt0391-286	journal	March 1991
Methods for measuring cellulase activities Wood, Thomas M.; Bhat, K. Mahalingeshwara Methods in Enzymology, Vol. 160, p. 87-112 https://doi.org/10.1016/0076-6879(88)60109-1	journal	January 1988
Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning Brumbauer, Anikó; Johansson, Göte; Réczey, Kati Bioseparation, Vol. 7, Issue 6, p. 287-295 https://doi.org/10.1023/A:1008153418019	journal	November 1999
Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei Te'o, Valentino S.J.; Cziferszky, Angela E.; Bergquist, Peter L. FEMS Microbiology Letters, Vol. 190, Issue 1, p. 13-19 https://doi.org/10.1111/j.1574-6968.2000.tb09255.x	journal	September 2000
Separation of endo- and exo-type cellulases using a new affinity chromatography method van Tilbeurgh, Herman; Bhikhabhai, Ramagauri; Göran Pettersson, L. FEBS Letters, Vol. 169, Issue 2, p. 215-218 https://doi.org/10.1016/0014-5793(84)80321-X	journal	April 1984
Nucleotide sequence of the triosephosphate isomerase gene from Aspergillus nidulans: Implications for a differential loss of introns McKnight, Gary L.; O'Hara, Patrick J.; Parker, Michael L. Cell, Vol. 46, Issue 1, p. 143-147 https://doi.org/10.1016/0092-8674(86)90868-8	journal	July 1986
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA Zoller, Mark J.; Smith, Michael Nucleic Acids Research, Vol. 10, Issue 20, p. 6487-6500 https://doi.org/10.1093/nar/10.20.6487	journal	January 1982
Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae Penttilä, Merja E.; André, Lars; Lehtovaara, Päivi Gene, Vol. 63, Issue 1, p. 103-112 https://doi.org/10.1016/0378-1119(88)90549-5	journal	March 1988
Efficient transformation of Dictyostelium discoideum amoebae. Barclay, S. L.; Meller, E. Molecular and Cellular Biology, Vol. 3, Issue 12, p. 2117-2130 https://doi.org/10.1128/MCB.3.12.2117	journal	December 1983
[79] Purification of all glycolytic enzymes from one muscle extract Scopes, Robert K.; Stoter, Ann Methods in Enzymology, Vol. 90, p. 479-490 https://doi.org/10.1016/S0076-6879(82)90175-6	journal	January 1982
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A. Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402 https://doi.org/10.1093/nar/25.17.3389	journal	September 1997
Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea Filho, Edivaldo Ximenes Ferreira Canadian Journal of Microbiology, Vol. 42, Issue 1, p. 1-5 https://doi.org/10.1139/m96-001	journal	January 1996
A novel genetic system to detect protein–protein interactions Fields, Stanley; Song, Ok-kyu Nature, Vol. 340, Issue 6230, p. 245-246 https://doi.org/10.1038/340245a0	journal	July 1989
Comparison of biosequences Smith, Temple F.; Waterman, Michael S. Advances in Applied Mathematics, Vol. 2, Issue 4, p. 482-489 https://doi.org/10.1016/0196-8858(81)90046-4	journal	December 1981
Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. Hu, Q. J.; Bautista, C.; Edwards, G. M. Molecular and Cellular Biology, Vol. 11, Issue 11, p. 5792-5799 https://doi.org/10.1128/MCB.11.11.5792	journal	November 1991
A radiation hybrid map of 18 growth factor, growth factor receptor, hormone receptor, or neurotransmitter receptor genes on the distal region of the long arm of chromosome 5 Warrington, J. A.; Bailey, S. K.; Armstrong, E. Genomics, Vol. 13, Issue 3, p. 803-808 https://doi.org/10.1016/0888-7543(92)90156-M	journal	July 1992
Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain Lehtio, Janne; Wernerus, Henrik; Samuelson, Patrik FEMS Microbiology Letters, Vol. 195, Issue 2, p. 197-204 https://doi.org/10.1111/j.1574-6968.2001.tb10521.x	journal	February 2001
Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies Nidetzky, Bernd; Claeyssens, Marc Biotechnology and Bioengineering, Vol. 44, Issue 8, p. 961-966 https://doi.org/10.1002/bit.260440812	journal	October 1994
Multiple Proline Substitutions Cumulatively Thermostabilize Bacillus Cereus ATCC7064 Oligo-1,6-Glucosidase. Irrefragable Proof Supporting the Proline Rule Watanabe, Kunihiko; Masuda, Tomoko; Ohashi, Hiroyuki European Journal of Biochemistry, Vol. 226, Issue 2, p. 277-283 https://doi.org/10.1111/j.1432-1033.1994.tb20051.x	journal	December 1994
Protein tolerance to random amino acid change Guo, H. H.; Choe, J.; Loeb, L. A. Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210 https://doi.org/10.1073/pnas.0403255101	journal	June 2004
Improved oligonudeotide site-directed rautagenesis using M13 vectors Carter, Paul; Bedouelle, Hugues; Winter, Greg Nucleic Acids Research, Vol. 13, Issue 12, p. 4431-4443 https://doi.org/10.1093/nar/13.12.4431	journal	January 1985
Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis TOMME, Peter; TILBEURGH, Herman; PETTERSSON, Goran European Journal of Biochemistry, Vol. 170, Issue 3, p. 575-581 https://doi.org/10.1111/j.1432-1033.1988.tb13736.x	journal	January 1988
Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae Sakamoto, S.; Tamura, G.; Ito, K. Current Genetics, Vol. 27, Issue 5, p. 435-439 https://doi.org/10.1007/BF00311212	journal	April 1995
The roles and function of cellulose-binding domains Linder, Markus; Teeri, Tuula T. Journal of Biotechnology, Vol. 57, Issue 1-3, p. 15-28 https://doi.org/10.1016/S0168-1656(97)00087-4	journal	September 1997
Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose Srisodsuk, Malee; Lehtiö, Janne; Linder, Markus Journal of Biotechnology, Vol. 57, Issue 1-3, p. 49-57 https://doi.org/10.1016/S0168-1656(97)00088-6	journal	September 1997
Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast Hari Krishna, S.; Janardhan Reddy, T.; Chowdary, G. V. Bioresource Technology, Vol. 77, Issue 2, p. 193-196 https://doi.org/10.1016/S0960-8524(00)00151-6	journal	April 2001
Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase Goedegebuur, Frits; Fowler, Timothy; Phillips, Jay Current Genetics, Vol. 41, Issue 2, p. 89-98 https://doi.org/10.1007/s00294-002-0290-2	journal	May 2002
The molecular biology of the yeast saccharomyces Gichner, T. Biologia Plantarum, Vol. 24, Issue 5, p. 340-340 https://doi.org/10.1007/BF02909099	journal	September 1982
Contribution of hydrophobic interactions to protein stability Kellis, James T.; Nyberg, Kerstin; S˘ail, Das̆a Nature, Vol. 333, Issue 6175, p. 784-786 https://doi.org/10.1038/333784a0	journal	June 1988
Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans Lockington, Robin A.; Scaly-Lewis, H. M.; Scazzocchio, C. Gene, Vol. 33, Issue 2, p. 137-149 https://doi.org/10.1016/0378-1119(85)90088-5	journal	January 1985
Genetic transformation of intact cells of Bacillus subtilis by electroporation Brigidi, P.; De Rossi, E.; Bertarini, M. L. FEMS Microbiology Letters, Vol. 67, Issue 1-2, p. 135-138 https://doi.org/10.1016/0378-1097(90)90182-P	journal	January 1990
cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast Saloheimo, Markku; Nakari-SetaLa, Tiina; Tenkanen, Maija European Journal of Biochemistry, Vol. 249, Issue 2, p. 584-591 https://doi.org/10.1111/j.1432-1033.1997.00584.x	journal	October 1997
Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography Ellouz, S.; Durand, H.; Tiraby, G. Journal of Chromatography A, Vol. 396, p. 307-317 https://doi.org/10.1016/S0021-9673(01)94068-3	journal	January 1987
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva Biochemistry, Vol. 38, Issue 36, p. 11643-11650 https://doi.org/10.1021/bi990993h	journal	September 1999
Production of fully human antibodies by transgenic mice Jakobovits, Aya Current Opinion in Biotechnology, Vol. 6, Issue 5, p. 561-566 https://doi.org/10.1016/0958-1669(95)80093-X	journal	January 1995
Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae Rothstein, Steven J.; Lahners, Kristine N.; Lazarus, Colin M. Gene, Vol. 55, Issue 2-3, p. 353-356 https://doi.org/10.1016/0378-1119(87)90296-4	journal	January 1987
EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme Saloheimo, M.; Lehtovaara, P.; Penttilä, M. Gene, Vol. 63, Issue 1, p. 11-21 https://doi.org/10.1016/0378-1119(88)90541-0	journal	March 1988
Properties of cellulolytic enzyme systems Wood, Thomas M. Biochemical Society Transactions, Vol. 13, Issue 2, p. 407-410 https://doi.org/10.1042/bst0130407	journal	April 1985
Reshaping human antibodies: grafting an antilysozyme activity Verhoeyen, M.; Milstein, C.; Winter, G. Science, Vol. 239, Issue 4847, p. 1534-1536 https://doi.org/10.1126/science.2451287	journal	March 1988
Replacing the complementarity-determining regions in a human antibody with those from a mouse Jones, Peter T.; Dear, Paul H.; Foote, Jefferson Nature, Vol. 321, Issue 6069, p. 522-525 https://doi.org/10.1038/321522a0	journal	May 1986
Purification and properties of an extracellular β-glucosidase from Lenzites trabea Herr, D.; Baumer, F.; Dellweg, H. European Journal of Applied Microbiology and Biotechnology, Vol. 5, Issue 1, p. 29-36 https://doi.org/10.1007/BF00515684	journal	January 1978
Determinants of Enzyme Thermostability Observed in the Molecular Structure of Thermus aquaticus d -Glyceraldehyde-3-phosphate Dehydrogenase at 2.5 Å Resolution Tanner, John J.; Hecht, Ralph M.; Krause, Kurt L. Biochemistry, Vol. 35, Issue 8, p. 2597-2609 https://doi.org/10.1021/bi951988q	journal	January 1996
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids Broun, Pierre; Shanklin, John; Whittle, Ed Science, Vol. 282, Issue 5392, p. 1315-1317 https://doi.org/10.1126/science.282.5392.1315	journal	November 1998
Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae Cummings, C.; Fowler, T. Current Genetics, Vol. 29, Issue 3, p. 227-233 https://doi.org/10.1007/BF02221552	journal	February 1996
The plasmid replicator AMA1 in Aspergillus nidulans is an inverted duplication of a low-copy-number dispersed genomic repeat Aleksenko, Alexei; Clutterbuck, A. John Molecular Microbiology, Vol. 19, Issue 3, p. 565-574 https://doi.org/10.1046/j.1365-2958.1996.400937.x	journal	February 1996
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J. Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680 https://doi.org/10.1093/nar/22.22.4673	journal	January 1994
Kinetics of cellulose saccharification by Trichoderma reesei cellulases Ortega, N. International Biodeterioration & Biodegradation, Vol. 47, Issue 1, p. 7-14 https://doi.org/10.1016/S0964-8305(00)00101-3	journal	January 2001
Purification and characterization of two extracellular β-glucosidases from Trichoderma reesei Chen, Huizhong; Hayn, Marianne; Esterbauer, Hermann Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1121, Issue 1-2, p. 54-60 https://doi.org/10.1016/0167-4838(92)90336-C	journal	May 1992
Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes Bergès, Thierry; Barreau, Christian Current Genetics, Vol. 19, Issue 5, p. 359-365 https://doi.org/10.1007/BF00309596	journal	May 1991
Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA Lorito, M.; Hayes, C. K.; Di Pietro, A. Current Genetics, Vol. 24, Issue 4, p. 349-356 https://doi.org/10.1007/BF00336788	journal	October 1993
Construction of mutant and chimeric genes using the polymerase chain reaction Vallette, Francois; Mege, Emmanuelle; Reiss, Allison Nucleic Acids Research, Vol. 17, Issue 2, p. 723-733 https://doi.org/10.1093/nar/17.2.723	journal	January 1989
Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus Kawaguchi, Takashi; Enoki, Tatsuji; Tsurumaki, Shinji Gene, Vol. 173, Issue 2, p. 287-288 https://doi.org/10.1016/0378-1119(96)00179-5	journal	September 1996
Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations Sheir-Neiss, G.; Montenecourt, B. S. Applied Microbiology and Biotechnology, Vol. 20, Issue 1, p. 46-53 https://doi.org/10.1007/BF00254645	journal	July 1984
Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei: Separation of functional domains Van Tilbeurgh, Herman; Tomme, Peter; Claeyssens, Marc FEBS Letters, Vol. 204, Issue 2, p. 223-227 https://doi.org/10.1016/0014-5793(86)80816-X	journal	August 1986
Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. Hynes, M. J.; Corrick, C. M.; King, J. A. Molecular and Cellular Biology, Vol. 3, Issue 8, p. 1430-1439 https://doi.org/10.1128/MCB.3.8.1430	journal	August 1983
Development of a homologous transformation system for Aspergillus niger based on the pyrG gene van Hartingsveldt, Wim; Mattern, Ineke E.; van Zeijl, Cora M. J. Molecular and General Genetics MGG, Vol. 206, Issue 1, p. 71-75 https://doi.org/10.1007/BF00326538	journal	January 1987
Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae Panttilä, Merja E.; André, Lars; Saloheimo, Markku Yeast, Vol. 3, Issue 3, p. 175-185 https://doi.org/10.1002/yea.320030305	journal	September 1987
Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography Medve, József; Lee, Dora; Tjerneld, Folke Journal of Chromatography A, Vol. 808, Issue 1-2, p. 153-165 https://doi.org/10.1016/S0021-9673(98)00132-0	journal	May 1998
Reshaping human antibodies for therapy Riechmann, Lutz; Clark, Michael; Waldmann, Herman Nature, Vol. 332, Issue 6162, p. 323-327 https://doi.org/10.1038/332323a0	journal	March 1988
Engineering thermostability: lessons from thermophilic proteins Russell, Rupert J. M.; Taylor, Garry L. Current Opinion in Biotechnology, Vol. 6, Issue 4, p. 370-374 https://doi.org/10.1016/0958-1669(95)80064-6	journal	January 1995
A comparison of gel-based, nylon filter and microarray techniques to detect differential RNA expression in plants Baldwin, Don; Crane, Virginia; Rice, Douglas Current Opinion in Plant Biology, Vol. 2, Issue 2, p. 96-103 https://doi.org/10.1016/S1369-5266(99)80020-X	journal	April 1999
New families in the classification of glycosyl hydrolases based on amino acid sequence similarities Henrissat, B.; Bairoch, A. Biochemical Journal, Vol. 293, Issue 3, p. 781-788 https://doi.org/10.1042/bj2930781	journal	August 1993
Production of Antigen-Specific Human Antibodies from Mice Engineered with Human Heavy and Light Chain YACs Jakobovits, Aya; Green, Larry L.; Hardy, Margaret C. Annals of the New York Academy of Sciences, Vol. 764, Issue 1, p. 525-535 https://doi.org/10.1111/j.1749-6632.1995.tb55878.x	journal	September 1995
Urea and Thermal Equilibrium Denaturation Studies on the Dimerization Domain of Escherichia coli Trp Repressor Gloss, Lisa M.; Matthews, C. Robert Biochemistry, Vol. 36, Issue 19, p. 5612-5623 https://doi.org/10.1021/bi970056e	journal	May 1997
Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II Teeri, Tuula T.; Lehtovaara, Päivi; Kauppinen, Sakari Gene, Vol. 51, Issue 1, p. 43-52 https://doi.org/10.1016/0378-1119(87)90472-0	journal	January 1987
Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp Suurnäkki, A.; Tenkanen, M.; Siika-aho, M. Cellulose, Vol. 7, Issue 2, p. 189-209 https://doi.org/10.1023/A:1009280109519	journal	June 2000
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei Penttilä, Merja; Nevalainen, Helena; Rättö, Marjaana Gene, Vol. 61, Issue 2, p. 155-164 https://doi.org/10.1016/0378-1119(87)90110-7	journal	January 1987
Site-directed mutagenesis of the putative catalytic residues of Trichoderma reesci cellobiohydrolase I and endoglucanase I Mitsuishi, Yasushi; Nitisinprasert, Sunee; Saloheimo, Markku FEBS Letters, Vol. 275, Issue 1-2, p. 135-138 https://doi.org/10.1016/0014-5793(90)81457-Y	journal	November 1990
Molecular Biology of Cellulolytic Fungi Nevalainen, H.; Penttilä, M.; Kück, Ulrich Genetics and Biotechnology, p. 303-319 https://doi.org/10.1007/978-3-662-10364-7_18	book	January 1995
Continuous cultures of fused cells secreting antibody of predefined specificity Köhler, G.; Milstein, C. Nature, Vol. 256, Issue 5517, p. 495-497 https://doi.org/10.1038/256495a0	journal	August 1975
Chapter 43 Rethinking Your Purification Procedure Deutscher, Murray P. Guide to Protein Purification, 2nd Edition https://doi.org/10.1016/S0076-6879(09)63043-3	book	January 2009
ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei Aro, Nina; Saloheimo, Anu; Ilmén, Marja Journal of Biological Chemistry, Vol. 276, Issue 26, p. 24309-24314 https://doi.org/10.1074/jbc.M003624200	journal	April 2001
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different Seffernick, J. L.; de Souza, M. L.; Sadowsky, M. J. Journal of Bacteriology, Vol. 183, Issue 8, p. 2405-2410 https://doi.org/10.1128/JB.183.8.2405-2410.2001	journal	April 2001
Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori. Nunberg, J. H.; Meade, J. H.; Cole, G. Molecular and Cellular Biology, Vol. 4, Issue 11, p. 2306-2315 https://doi.org/10.1128/MCB.4.11.2306	journal	November 1984
Structural and Genetic Analysis of Protein Stability Matthews, Brian W. Annual Review of Biochemistry, Vol. 62, Issue 1, p. 139-160 https://doi.org/10.1146/annurev.bi.62.070193.001035	journal	June 1993
Heterologous gene expression in filamentous fungi Saunders, Gunter; Picknett, Tessa M.; Tuite, Michael F. Trends in Biotechnology, Vol. 7, Issue 10, p. 283-287 https://doi.org/10.1016/0167-7799(89)90048-6	journal	October 1989
A general method applicable to the search for similarities in the amino acid sequence of two proteins Needleman, Saul B.; Wunsch, Christian D. Journal of Molecular Biology, Vol. 48, Issue 3, p. 443-453 https://doi.org/10.1016/0022-2836(70)90057-4	journal	March 1970
Enzymic activities of endo-1,4-β-d-glucanases purified from Trichoderma viride Shoemaker, S. P.; Brown, R. D. Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 523, Issue 1, p. 133-146 https://doi.org/10.1016/0005-2744(78)90016-5	journal	March 1978
Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase Campbell, Edward I.; Unkles, Shiela E.; Macro, Janet A. Current Genetics, Vol. 16, Issue 1, p. 53-56 https://doi.org/10.1007/BF00411084	journal	July 1989
Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans Timberlake, William E.; Barnard, Elizabeth C. Cell, Vol. 26, Issue 1, p. 29-37 https://doi.org/10.1016/0092-8674(81)90030-1	journal	October 1981
Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant Becker, Dieter; Braet, Christophe; Brumer, Harry Biochemical Journal, Vol. 356, Issue 1, p. 19-30 https://doi.org/10.1042/bj3560019	journal	May 2001
Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction Tomaz, Cândida T.; Queiroz, João A. Journal of Chromatography A, Vol. 865, Issue 1-2, p. 123-128 https://doi.org/10.1016/S0021-9673(99)00851-1	journal	December 1999
Importance of Hydrogen-Bond Formation in Stabilizing the Transition State of Subtilisin Wells, J. A.; Cunningham, B. C.; Graycar, T. P. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 317, Issue 1540, p. 415-423 https://doi.org/10.1098/rsta.1986.0051	journal	April 1986
Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites Wells, James A.; Vasser, Mark; Powers, David B. Gene, Vol. 34, Issue 2-3, p. 315-323 https://doi.org/10.1016/0378-1119(85)90140-4	journal	January 1985
Transformation of Trichoderma harzianum by high-voltage electric pulse Goldman, G. H.; Van Montagu, M.; Herrera-Estrella, A. Current Genetics, Vol. 17, Issue 2, p. 169-174 https://doi.org/10.1007/BF00312863	journal	February 1990
Cellulases of Trichoderma reesei Schülein, Martin Methods in Enzymology, Vol. 160, p. 234-242 https://doi.org/10.1016/0076-6879(88)60125-X	journal	January 1988
Temperature adaptation of lactate dehydrogenase Structural, functional and genetic aspects Zuber, H. Biophysical Chemistry, Vol. 29, Issue 1-2, p. 171-179 https://doi.org/10.1016/0301-4622(88)87037-6	journal	February 1988
Characteristics of fungal cellulases Goyal, Anil; Ghosh, Bijan; Eveleigh, Douglas Bioresource Technology, Vol. 36, Issue 1, p. 37-50 https://doi.org/10.1016/0960-8524(91)90098-5	journal	January 1991
Genetically engineered peptide fusions for improved protein partitioning in aqueous two-phase systems Collén, Anna; Ward, Michael; Tjerneld, Folke Journal of Chromatography A, Vol. 910, Issue 2, p. 275-284 https://doi.org/10.1016/S0021-9673(00)01212-7	journal	March 2001
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins Ward, Michael; Wilson, Lori J.; Kodama, Katherine H. Applied Microbiology and Biotechnology, Vol. 39, Issue 6, p. 738-743 https://doi.org/10.1007/BF00164459	journal	August 1993
Cellulase families and their genes Knowles, Jonathan; Lehtovaara, Päivi; Teeri, Tuula Trends in Biotechnology, Vol. 5, Issue 9, p. 255-261 https://doi.org/10.1016/0167-7799(87)90102-8	journal	September 1987
Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12 Halldórsdóttir, S.; Thórólfsdót, E. T.; Spilliaert, R. Applied Microbiology and Biotechnology, Vol. 49, Issue 3, p. 277-284 https://doi.org/10.1007/s002530051169	journal	March 1998
Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27 Shoemaker, S.; Schweickart, V.; Ladner, M. Nature Biotechnology, Vol. 1, Issue 8, p. 691-696 https://doi.org/10.1038/nbt1083-691	journal	October 1983
CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora Saarilahti, Hannu T.; Henrissat, Bernard; Tapio Palva, E. Gene, Vol. 90, Issue 1, p. 9-14 https://doi.org/10.1016/0378-1119(90)90433-R	journal	May 1990
Characterization of cellulases by HPLC separation Fliess, A.; Schügerl, K. European Journal of Applied Microbiology and Biotechnology, Vol. 17, Issue 5, p. 314-318 https://doi.org/10.1007/BF00508028	journal	January 1983

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