Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

CBH1 homologs and variant CBH1 cellulases

Patent ·
OSTI ID:1016827
Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
Research Organization:
Midwest Research Institute
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-99GO10337
Assignee:
GFO
Patent Number(s):
7951570
Application Number:
12/250,227
OSTI ID:
1016827
Country of Publication:
United States
Language:
English

References (121)

Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies journal October 1994
Two different types of intervening sequences in the glucoamylase gene from Aspergillus niger. journal July 1984
Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. journal November 1996
Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae journal September 1987
Gene Transfer and Expression book January 1990
Molecular Biology of Cellulolytic Fungi book January 1995
Viruses and gene silencing in plants book January 1999
Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins journal August 1993
Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations journal July 1984
Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes journal May 1991
Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae journal April 1995
Transformation of Trichoderma harzianum by high-voltage electric pulse journal February 1990
Development of a homologous transformation system for Aspergillus niger based on the pyrG gene journal January 1987
Primary structure of the trpC gene from Aspergillus nidulans journal January 1985
Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA journal October 1993
Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase journal July 1989
Characterization of cellulases by HPLC separation journal January 1983
Purification and properties of an extracellular β-glucosidase from Lenzites trabea journal January 1978
Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12 journal March 1998
Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase journal May 2002
Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae journal February 1996
Developments in Directed Evolution for Improving Enzyme Functions journal August 2007
Enzymic activities of endo-1,4-β-d-glucanases purified from Trichoderma viride journal March 1978
Separation of endo- and exo-type cellulases using a new affinity chromatography method journal April 1984
Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei: Separation of functional domains journal August 1986
Site-directed mutagenesis of the putative catalytic residues of Trichoderma reesci cellobiohydrolase I and endoglucanase I journal November 1990
A general method applicable to the search for similarities in the amino acid sequence of two proteins journal March 1970
Methods for measuring cellulase activities journal January 1988
Cellulases of Trichoderma reesei journal January 1988
[57] Rethinking your purification procedure book January 1990
Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans journal October 1981
Nucleotide sequence of the triosephosphate isomerase gene from Aspergillus nidulans: Implications for a differential loss of introns journal July 1986
Purification and characterization of two extracellular β-glucosidases from Trichoderma reesei journal May 1992
Cellulase families and their genes journal September 1987
Comparison of biosequences journal December 1981
Temperature adaptation of lactate dehydrogenase Structural, functional and genetic aspects journal February 1988
Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans journal January 1985
Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites journal January 1985
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei journal January 1987
Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae journal January 1987
Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II journal January 1987
EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme journal March 1988
Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae journal March 1988
CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora journal May 1990
Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus journal September 1996
A radiation hybrid map of 18 growth factor, growth factor receptor, hormone receptor, or neurotransmitter receptor genes on the distal region of the long arm of chromosome 5 journal July 1992
Engineering thermostability: lessons from thermophilic proteins journal January 1995
Production of fully human antibodies by transgenic mice journal January 1995
Characteristics of fungal cellulases journal January 1991
Heterologous Gene Expression in Filamentous Fungi book December 1990
Recombinant PCR book December 1989
Genetically engineered peptide fusions for improved protein partitioning in aqueous two-phase systems journal March 2001
Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography journal January 1987
Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography journal May 1998
Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction journal December 1999
Basic local alignment search tool journal October 1990
[79] Purification of all glycolytic enzymes from one muscle extract journal January 1982
The roles and function of cellulose-binding domains journal September 1997
Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose journal September 1997
Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain journal February 2001
Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast journal April 2001
Kinetics of cellulose saccharification by Trichoderma reesei cellulases journal January 2001
A comparison of gel-based, nylon filter and microarray techniques to detect differential RNA expression in plants journal April 1999
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design journal August 2005
Prediction of protein function from protein sequence and structure journal January 1999
Detection of a Stable Intermediate in the Thermal Unfolding of a Cysteine-Free Form of Dihydrofolate Reductase from Escherichia Coli journal August 1995
Determinants of Enzyme Thermostability Observed in the Molecular Structure of Thermus aquaticus d -Glyceraldehyde-3-phosphate Dehydrogenase at 2.5 Å Resolution journal January 1996
Urea and Thermal Equilibrium Denaturation Studies on the Dimerization Domain of Escherichia coli Trp Repressor journal May 1997
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal September 1999
Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning journal November 1999
Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp journal June 2000
Continuous cultures of fused cells secreting antibody of predefined specificity journal August 1975
Replacing the complementarity-determining regions in a human antibody with those from a mouse journal May 1986
Reshaping human antibodies for therapy journal March 1988
Contribution of hydrophobic interactions to protein stability journal June 1988
A novel genetic system to detect protein–protein interactions journal July 1989
A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I journal March 1991
Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27 journal October 1983
New families in the classification of glycosyl hydrolases based on amino acid sequence similarities journal August 1993
Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant journal May 2001
Properties of cellulolytic enzyme systems journal April 1985
The plasmid replicator AMA1 in Aspergillus nidulans is an inverted duplication of a low-copy-number dispersed genomic repeat journal February 1996
Protein tolerance to random amino acid change journal June 2004
Transformation of Aspergillus nidulans by using a trpC plasmid. journal March 1984
Rapid and efficient site-specific mutagenesis without phenotypic selection. journal January 1985
Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding. journal October 1987
Improved tools for biological sequence comparison. journal April 1988
Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor. journal September 1991
Amino acid substitution matrices from protein blocks. journal November 1992
Applications and statistics for multiple high-scoring segments in molecular sequences. journal June 1993
ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei journal April 2001
Structure of Cellulases and Their Applications journal April 1997
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA journal January 1982
Improved oligonudeotide site-directed rautagenesis using M13 vectors journal January 1985
Construction of mutant and chimeric genes using the polymerase chain reaction journal January 1989
Nucleotide sequence of the cellobiohydrolase gene from Trichoderma viride journal January 1990
Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase) journal January 1990
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice journal January 1994
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs journal September 1997
Importance of Hydrogen-Bond Formation in Stabilizing the Transition State of Subtilisin
  • Wells, J. A.; Cunningham, B. C.; Graycar, T. P.
  • Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 317, Issue 1540, p. 415-423 https://doi.org/10.1098/rsta.1986.0051
journal April 1986
Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis journal January 1988
Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli journal September 1994
Multiple Proline Substitutions Cumulatively Thermostabilize Bacillus Cereus ATCC7064 Oligo-1,6-Glucosidase. Irrefragable Proof Supporting the Proline Rule journal December 1994
cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast journal October 1997
Genetic transformation of intact cells of Bacillus subtilis by electroporation journal January 1990
Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei journal September 2000
Production of Antigen-Specific Human Antibodies from Mice Engineered with Human Heavy and Light Chain YACs journal September 1995
Response of a protein structure to cavity-creating mutations and its relation to the hydrophobic effect journal January 1992
Reshaping human antibodies: grafting an antilysozyme activity journal March 1988
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids journal November 1998
Aromatic-aromatic interaction: a mechanism of protein structure stabilization journal July 1985
Structure, organization, and transcription of a cellobiohydrolase gene cluster from Phanerochaete chrysosporium. journal January 1992
Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae. journal January 1996
Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. journal January 1997
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal April 2001
Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. journal November 1991
Efficient transformation of Dictyostelium discoideum amoebae. journal December 1983
Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. journal August 1983
Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori. journal November 1984
Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea journal January 1996
Structural and Genetic Analysis of Protein Stability journal June 1993

Similar Records

CBH1 homologs and variant CBH1 cellulases
Patent · Mon Nov 17 23:00:00 EST 2008 · OSTI ID:986212

CBH1 homologs and varian CBH1 cellulase
Patent · Tue Jul 01 00:00:00 EDT 2014 · OSTI ID:1136635

Variants of cellobiohydrolases
Patent · Mon Apr 09 20:00:00 EDT 2018 · OSTI ID:1434527

Related Subjects