Photobiological Hydrogen Research
- Florida International Univ. (FIU), Miami, FL (United States); Florida International University (FIU)
- Florida International Univ. (FIU), Miami, FL (United States)
The project objectives are to develop bio-hydrogen production by: Cloning the structural and subunit genes (cooKMUX and cooLH resp.) of the O2-tolerant NiFe-hydrogenase from the photosynthetic bacterium Rubrivivax gelatinosus CBS strain in collaboration with NREL. Cloning the active site maturation genes (hypA-F) of the CBS hydrogenase in collaboration with NREL. Transforming the structural and subunits genes, along with the maturation genes, into E. coli and determining the minimum number of genes required for expression of a functional hydrogenase. Upon expression of a functional hydrogenase, purifying and characterizing the recombinant hydrogenase from E. coli and performing bioreactor studies to optimize hydrogen production by E. coli.
- Research Organization:
- Florida International Univ. (FIU), Miami, FL (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG36-06GO86047
- OSTI ID:
- 1130089
- Report Number(s):
- DOE-FIU--86047
- Country of Publication:
- United States
- Language:
- English
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