Genome annotation provides insight into carbon monoxide and hydrogen metabolism in Rubrivivax gelatinosus

Wawrousek, Karen; Noble, Scott; Korlach, Jonas; Chen, Jin; Eckert, Carrie; Yu, Jianping; Maness, Pin -Ching

doi:10.1371/journal.pone.0114551

Genome annotation provides insight into carbon monoxide and hydrogen metabolism in Rubrivivax gelatinosus

Journal Article · Fri Dec 05 00:00:00 EST 2014 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0114551· OSTI ID:1169210

Wawrousek, Karen ^[1]; Noble, Scott ^[2]; Korlach, Jonas ^[3]; Chen, Jin ^[4]; Eckert, Carrie ^[1]; Yu, Jianping ^[1]; Maness, Pin -Ching ^[1]

Univ. of Wyoming, Laramie, WY (United States). Dept. of Chemical and Petroleum Engineering
National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center
Pacific Biosciences, Menlo Park, CA (United States
Michigan State Univ., East Lansing, MI (United States). Dept. of Energy Plant Research Lab.

In this article, we report here the sequencing and analysis of the genome of the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS. This microbe is a model for studies of its carboxydotrophic life style under anaerobic condition, based on its ability to utilize carbon monoxide (CO) as the sole carbon substrate and water as the electron acceptor, yielding CO₂ and H₂ as the end products. The CO-oxidation reaction is known to be catalyzed by two enzyme complexes, the CO dehydrogenase and hydrogenase. As expected, analysis of the genome of Rx. gelatinosus CBS reveals the presence of genes encoding both enzyme complexes. The CO-oxidation reaction is CO-inducible, which is consistent with the presence of two putative CO-sensing transcription factors in its genome. Genome analysis also reveals the presence of two additional hydrogenases, an uptake hydrogenase that liberates the electrons in H₂ in support of cell growth, and a regulatory hydrogenase that senses H₂ and relays the signal to a two-component system that ultimately controls synthesis of the uptake hydrogenase. The genome also contains two sets of hydrogenase maturation genes which are known to assemble the catalytic metallocluster of the hydrogenase NiFe active site. Finally and collectively, the genome sequence and analysis information reveals the blueprint of an intricate network of signal transduction pathways and its underlying regulation that enables Rx. gelatinosus CBS to thrive on CO or H₂ in support of cell growth.

View Accepted Manuscript (DOE)

Research Organization:: NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Fuel Cell Technologies Program (EE-3F); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1169210

Report Number(s):: NREL/JA-2700-61988

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 12 Vol. 9; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (76)

Hydrogenase Adams, Michael W. W.; Mortenson, Leonard E.; Chen, Jiann-Shin Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, Vol. 594, Issue 2-3, p. 105-176 https://doi.org/10.1016/0304-4173(80)90007-5	journal	December 1980
CO-dependent H2 evolution by Rhodospirillum rubrum: Role of CODH:CooF complex Singer, Steven W.; Hirst, Marissa B.; Ludden, Paul W. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1757, Issue 12 https://doi.org/10.1016/j.bbabio.2006.10.003	journal	December 2006
EMBOSS: The European Molecular Biology Open Software Suite Rice, Peter; Longden, Ian; Bleasby, Alan Trends in Genetics, Vol. 16, Issue 6 https://doi.org/10.1016/s0168-9525(00)02024-2	journal	June 2000
Hydrogen production from a carbon-monoxide oxidation pathway in Rubrivivax gelatinosus Maness, P. International Journal of Hydrogen Energy, Vol. 27, Issue 11-12 https://doi.org/10.1016/s0360-3199(02)00107-6	journal	November 2002
The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre Fritsch, Johannes; Scheerer, Patrick; Frielingsdorf, Stefan Nature, Vol. 479, Issue 7372 https://doi.org/10.1038/nature10505	journal	October 2011
A hybrid approach for the automated finishing of bacterial genomes Bashir, Ali; Klammer, Aaron A.; Robins, William P. Nature Biotechnology, Vol. 30, Issue 7 https://doi.org/10.1038/nbt.2288	journal	July 2012
Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16 Pohlmann, Anne; Fricke, Wolfgang Florian; Reinecke, Frank Nature Biotechnology, Vol. 24, Issue 10 https://doi.org/10.1038/nbt1244	journal	September 2006
An Escherichia coli hydrogenase-3-type hydrogenase in methanogenic archaea Kunkel, Andreas; Vorholt, Julia A.; Thauer, Rudolf K. European Journal of Biochemistry, Vol. 252, Issue 3 https://doi.org/10.1046/j.1432-1327.1998.2520467.x	journal	March 1998
Redox-dependent activation of CO dehydrogenase from Rhodospirillum rubrum Heo, J.; Halbleib, C. M.; Ludden, P. W. Proceedings of the National Academy of Sciences, Vol. 98, Issue 14 https://doi.org/10.1073/pnas.141230698	journal	June 2001
Photoactive yellow protein: A structural prototype for the three-dimensional fold of the PAS domain superfamily Pellequer, J. -L.; Wager-Smith, K. A.; Kay, S. A. Proceedings of the National Academy of Sciences, Vol. 95, Issue 11 https://doi.org/10.1073/pnas.95.11.5884	journal	May 1998
Characterization of a CO-responsive Transcriptional Activator from Rhodospirillum rubrum He, Yiping; Shelver, Daniel; Kerby, Robert L. Journal of Biological Chemistry, Vol. 271, Issue 1 https://doi.org/10.1074/jbc.271.1.120	journal	January 1996
Purification and Characterization of Membrane-associated CooC Protein and Its Functional Role in the Insertion of Nickel into Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum Jeon, Won Bae; Cheng, Jiujun; Ludden, Paul W. Journal of Biological Chemistry, Vol. 276, Issue 42 https://doi.org/10.1074/jbc.m104945200	journal	August 2001
Rubredoxin-related Maturation Factor Guarantees Metal Cofactor Integrity during Aerobic Biosynthesis of Membrane-bound [NiFe] Hydrogenase Fritsch, Johannes; Siebert, Elisabeth; Priebe, Jacqueline Journal of Biological Chemistry, Vol. 289, Issue 11 https://doi.org/10.1074/jbc.m113.544668	journal	March 2014
A novel type of conserved DNA-binding domain in the transcriptional regulators of the AlgR/AgrA/LytR family Nikolskaya, A. N. Nucleic Acids Research, Vol. 30, Issue 11 https://doi.org/10.1093/nar/30.11.2453	journal	June 2002
A flexible and efficient template format for circular consensus sequencing and SNP detection Travers, K. J.; Chin, C. -S.; Rank, D. R. Nucleic Acids Research, Vol. 38, Issue 15 https://doi.org/10.1093/nar/gkq543	journal	June 2010
A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis Soboh, B. Microbiology, Vol. 150, Issue 7 https://doi.org/10.1099/mic.0.27159-0	journal	July 2004
Molecular characterization of an operon ( hyp ) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli Lutz, S.; Jacobi, A.; Schlensog, V. Molecular Microbiology, Vol. 5, Issue 1 https://doi.org/10.1111/j.1365-2958.1991.tb01833.x	journal	January 1991
X-ray Crystal Structure of the Fe-Only Hydrogenase (CpI) from Clostridium pasteurianum to 1.8 Angstrom Resolution Peters, J. W. Science, Vol. 282, Issue 5395 https://doi.org/10.1126/science.282.5395.1853	journal	December 1998
Characterization of Genes Responsible for the CO-Linked Hydrogen Production Pathway in Rubrivivax gelatinosus Vanzin, G.; Yu, J.; Smolinski, S. Applied and Environmental Microbiology, Vol. 76, Issue 11 https://doi.org/10.1128/aem.02753-09	journal	April 2010
Characterization of the Oxygen Tolerance of a Hydrogenase Linked to a Carbon Monoxide Oxidation Pathway in Rubrivivax gelatinosus Maness, P. -C.; Smolinski, S.; Dillon, A. C. Applied and Environmental Microbiology, Vol. 68, Issue 6 https://doi.org/10.1128/aem.68.6.2633-2636.2002	journal	June 2002
Energy Generation from the CO Oxidation-Hydrogen Production Pathway in Rubrivivax gelatinosus Maness, P. -C.; Huang, J.; Smolinski, S. Applied and Environmental Microbiology, Vol. 71, Issue 6 https://doi.org/10.1128/aem.71.6.2870-2874.2005	journal	June 2005
RcoM: A New Single-Component Transcriptional Regulator of CO Metabolism in Bacteria Kerby, Robert L.; Youn, Hwan; Roberts, Gary P. Journal of Bacteriology, Vol. 190, Issue 9 https://doi.org/10.1128/jb.00033-08	journal	May 2008
Complete Genome Sequence of Phototrophic Betaproteobacterium Rubrivivax gelatinosus IL144 Nagashima, Sakiko; Kamimura, Akiko; Shimizu, Takayuki Journal of Bacteriology, Vol. 194, Issue 13 https://doi.org/10.1128/jb.00511-12	journal	July 2012
Draft Genome Sequence of Rubrivivax gelatinosus CBS Hu, P.; Lang, J.; Wawrousek, K. Journal of Bacteriology, Vol. 194, Issue 12 https://doi.org/10.1128/jb.00515-12	journal	May 2012
Sustaining N ₂ -Dependent Growth in the Presence of CO Kerby, Robert L.; Roberts, Gary P. Journal of Bacteriology, Vol. 193, Issue 3 https://doi.org/10.1128/jb.00794-10	journal	February 2011
Metabolism of carbon monoxide by Rhodopseudomonas gelatinosa: cell growth and properties of the oxidation system Uffen, R. L. Journal of Bacteriology, Vol. 155, Issue 3 https://doi.org/10.1128/jb.155.3.956-965.1983	journal	September 1983
Carbon monoxide dehydrogenase from Rhodospirillum rubrum Bonam, D.; Murrell, S. A.; Ludden, P. W. Journal of Bacteriology, Vol. 159, Issue 2 https://doi.org/10.1128/jb.159.2.693-699.1984	journal	August 1984
Membrane topography of anaerobic carbon monoxide oxidation in Rhodocyclus gelatinosus Champine, J. E.; Uffen, R. L. Journal of Bacteriology, Vol. 169, Issue 10 https://doi.org/10.1128/jb.169.10.4784-4789.1987	journal	October 1987
Regulation of carbon monoxide dehydrogenase and hydrogenase in Rhodospirillum rubrum: effects of CO and oxygen on synthesis and activity Bonam, D.; Lehman, L.; Roberts, G. P. Journal of Bacteriology, Vol. 171, Issue 6 https://doi.org/10.1128/jb.171.6.3102-3107.1989	journal	June 1989
Genetic and physiological characterization of the Rhodospirillum rubrum carbon monoxide dehydrogenase system. Kerby, R. L.; Hong, S. S.; Ensign, S. A. Journal of Bacteriology, Vol. 174, Issue 16 https://doi.org/10.1128/jb.174.16.5284-5294.1992	journal	January 1992
Carbon monoxide-induced activation of gene expression in Rhodospirillum rubrum requires the product of cooA, a member of the cyclic AMP receptor protein family of transcriptional regulators Shelver, D.; Kerby, R. L.; He, Y. Journal of Bacteriology, Vol. 177, Issue 8 https://doi.org/10.1128/jb.177.8.2157-2163.1995	journal	April 1995
Carbon monoxide-dependent growth of Rhodospirillum rubrum. Kerby, R. L.; Ludden, P. W.; Roberts, G. P. Journal of Bacteriology, Vol. 177, Issue 8 https://doi.org/10.1128/jb.177.8.2241-2244.1995	journal	April 1995
Characterization of the region encoding the CO-induced hydrogenase of Rhodospirillum rubrum. Fox, J. D.; He, Y.; Shelver, D. Journal of bacteriology, Vol. 178, Issue 21 https://doi.org/10.1128/jb.178.21.6200-6208.1996	journal	January 1996
Characterization of the CO-induced, CO-tolerant hydrogenase from Rhodospirillum rubrum and the gene encoding the large subunit of the enzyme. Fox, J. D.; Kerby, R. L.; Roberts, G. P. Journal of Bacteriology, Vol. 178, Issue 6 https://doi.org/10.1128/jb.178.6.1515-1524.1996	journal	March 1996
In vivo nickel insertion into the carbon monoxide dehydrogenase of Rhodospirillum rubrum: molecular and physiological characterization of cooCTJ. Kerby, R. L.; Ludden, P. W.; Roberts, G. P. Journal of Bacteriology, Vol. 179, Issue 7 https://doi.org/10.1128/jb.179.7.2259-2266.1997	journal	April 1997
How Photosynthetic Bacteria Harvest Solar Energy Cogdell, Richard J.; Isaacs, Neil W.; Howard, Tina D. Journal of Bacteriology, Vol. 181, Issue 13 https://doi.org/10.1128/jb.181.13.3869-3879.1999	journal	July 1999
Ni ²⁺ Transport and Accumulation in Rhodospirillum rubrum Watt, Richard K.; Ludden, Paul W. Journal of Bacteriology, Vol. 181, Issue 15 https://doi.org/10.1128/jb.181.15.4554-4560.1999	journal	August 1999
Energy-Converting [NiFe] Hydrogenases: More than Just H₂ Activation Hedderich, Reiner; Forzi, Lucia Journal of Molecular Microbiology and Biotechnology, Vol. 10, Issue 2-4 https://doi.org/10.1159/000091557	journal	January 2005
Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 Wu, Martin; Ren, Qinghu; Durkin, A. Scott PLoS Genetics, Vol. 1, Issue 5 https://doi.org/10.1371/journal.pgen.0010065	journal	January 2005
Carbon Metabolic Pathways in Phototrophic Bacteria and Their Broader Evolutionary Implications Tang, Kuo-Hsiang; Tang, Yinjie J.; Blankenship, Robert Eugene Frontiers in Microbiology, Vol. 2 https://doi.org/10.3389/fmicb.2011.00165	journal	January 2011
A Novel Heme Protein That Acts as a Carbon Monoxide-Dependent Transcriptional Activator inRhodospirillum rubrum Aono, Shigetoshi; Nakajima, Hiroshi; Saito, Kimio Biochemical and Biophysical Research Communications, Vol. 228, Issue 3 https://doi.org/10.1006/bbrc.1996.1727	journal	November 1996
Production of poly-3-hydroxyalkanoates from CO and H2 by a novel photosynthetic bacterium Maness, Pin-Ching; Weaver, Paul F. Applied Biochemistry and Biotechnology, Vol. 45-46, Issue 1 https://doi.org/10.1007/BF02941814	journal	March 1994
Characterization of the CO oxidation/H2 evolution system of Rhodospirillum rubrum. Role of a 22-kDa iron-sulfur protein in mediating electron transfer between carbon monoxide dehydrogenase and hydrogenase. Ensign, S. A.; Ludden, P. W. Journal of Biological Chemistry, Vol. 266, Issue 27 https://doi.org/10.1016/S0021-9258(18)55283-2	journal	September 1991
Purification and characterization of carbon monoxide dehydrogenase, a nickel, zinc, iron-sulfur protein, from Rhodospirillum rubrum. Bonam, D.; Ludden, P. W. Journal of Biological Chemistry, Vol. 262, Issue 7 https://doi.org/10.1016/S0021-9258(18)61456-5	journal	March 1987
EMBOSS: The European Molecular Biology Open Software Suite Rice, Peter; Longden, Ian; Bleasby, Alan Trends in Genetics, Vol. 16, Issue 6 https://doi.org/10.1016/S0168-9525(00)02024-2	journal	June 2000
Hydrogen production from a carbon-monoxide oxidation pathway in Rubrivivax gelatinosus Maness, P. International Journal of Hydrogen Energy, Vol. 27, Issue 11-12 https://doi.org/10.1016/S0360-3199(02)00107-6	journal	November 2002
Hybrid error correction and de novo assembly of single-molecule sequencing reads Koren, Sergey; Schatz, Michael C.; Walenz, Brian P. Nature Biotechnology, Vol. 30, Issue 7 https://doi.org/10.1038/nbt.2280	journal	July 2012
Transcriptional regulation of the uptake [NiFe]hydrogenase genes in Rhodobacter capsulatus Vignais, P. M.; Elsen, S.; Colbeau, A. Biochemical Society Transactions, Vol. 33, Issue 1 https://doi.org/10.1042/BST0330028	journal	February 2005
Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans: CO-oxidizing:H2-evolving enzyme complex Soboh, Basem; Linder, Dietmar; Hedderich, Reiner European Journal of Biochemistry, Vol. 269, Issue 22 https://doi.org/10.1046/j.1432-1033.2002.03282.x	journal	October 2002
X-ray crystallographic and computational studies of the O2-tolerant [NiFe]-hydrogenase 1 from Escherichia coli Volbeda, A.; Amara, P.; Darnault, C. Proceedings of the National Academy of Sciences, Vol. 109, Issue 14 https://doi.org/10.1073/pnas.1119806109	journal	March 2012
Life on carbon monoxide: X-ray structure of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase Drennan, C. L.; Heo, J.; Sintchak, M. D. Proceedings of the National Academy of Sciences, Vol. 98, Issue 21 https://doi.org/10.1073/pnas.211429998	journal	October 2001
Anaerobic growth of a Rhodopseudomonas species in the dark with carbon monoxide as sole carbon and energy substrate. Uffen, R. L. Proceedings of the National Academy of Sciences, Vol. 73, Issue 9 https://doi.org/10.1073/pnas.73.9.3298	journal	September 1976
A novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus Lenz, O.; Friedrich, B. Proceedings of the National Academy of Sciences, Vol. 95, Issue 21 https://doi.org/10.1073/pnas.95.21.12474	journal	October 1998
Purification and Characterization of Membrane-associated CooC Protein and Its Functional Role in the Insertion of Nickel into Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum Jeon, Won Bae; Cheng, Jiujun; Ludden, Paul W. Journal of Biological Chemistry, Vol. 276, Issue 42 https://doi.org/10.1074/jbc.M104945200	journal	August 2001
Rubredoxin-related Maturation Factor Guarantees Metal Cofactor Integrity during Aerobic Biosynthesis of Membrane-bound [NiFe] Hydrogenase Fritsch, Johannes; Siebert, Elisabeth; Priebe, Jacqueline Journal of Biological Chemistry, Vol. 289, Issue 11 https://doi.org/10.1074/jbc.M113.544668	journal	March 2014
Oxygen-tolerant H2 Oxidation by Membrane-bound [NiFe] Hydrogenases of Ralstonia Species Ludwig, Marcus; Cracknell, James A.; Vincent, Kylie A. Journal of Biological Chemistry, Vol. 284, Issue 1 https://doi.org/10.1074/jbc.M803676200	journal	January 2009
The CoxD Protein of Oligotropha carboxidovorans Is a Predicted AAA+ ATPase Chaperone Involved in the Biogenesis of the CO Dehydrogenase [CuSMoO2] Cluster Pelzmann, Astrid; Ferner, Marion; Gnida, Manuel Journal of Biological Chemistry, Vol. 284, Issue 14 https://doi.org/10.1074/jbc.M805354200	journal	April 2009
Gepard: a rapid and sensitive tool for creating dotplots on genome scale Krumsiek, J.; Arnold, R.; Rattei, T. Bioinformatics, Vol. 23, Issue 8 https://doi.org/10.1093/bioinformatics/btm039	journal	February 2007
Classification and phylogeny of hydrogenases Vignais, Paulette M.; Billoud, Bernard; Meyer, Jacques FEMS Microbiology Reviews, Vol. 25, Issue 4, p. 455-501 https://doi.org/10.1111/j.1574-6976.2001.tb00587.x	journal	August 2001
Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants Forzi, Lucia; Koch, Jurgen; Guss, Adam M. FEBS Journal, Vol. 272, Issue 18 https://doi.org/10.1111/j.1742-4658.2005.04889.x	journal	September 2005
A Ni-Fe-Cu Center in a Bifunctional Carbon Monoxide Dehydrogenase/ Acetyl-CoA Synthase Doukov, T. I. Science, Vol. 298, Issue 5593 https://doi.org/10.1126/science.1075843	journal	October 2002
Characterization of Genes Responsible for the CO-Linked Hydrogen Production Pathway in Rubrivivax gelatinosus Vanzin, G.; Yu, J.; Smolinski, S. Applied and Environmental Microbiology, Vol. 76, Issue 11 https://doi.org/10.1128/AEM.02753-09	journal	April 2010
Characterization of the Oxygen Tolerance of a Hydrogenase Linked to a Carbon Monoxide Oxidation Pathway in Rubrivivax gelatinosus Maness, P. -C.; Smolinski, S.; Dillon, A. C. Applied and Environmental Microbiology, Vol. 68, Issue 6 https://doi.org/10.1128/AEM.68.6.2633-2636.2002	journal	June 2002
Energy Generation from the CO Oxidation-Hydrogen Production Pathway in Rubrivivax gelatinosus Maness, P. -C.; Huang, J.; Smolinski, S. Applied and Environmental Microbiology, Vol. 71, Issue 6 https://doi.org/10.1128/AEM.71.6.2870-2874.2005	journal	June 2005
RcoM: A New Single-Component Transcriptional Regulator of CO Metabolism in Bacteria Kerby, Robert L.; Youn, Hwan; Roberts, Gary P. Journal of Bacteriology, Vol. 190, Issue 9 https://doi.org/10.1128/JB.00033-08	journal	May 2008
Complete Genome Sequence of Phototrophic Betaproteobacterium Rubrivivax gelatinosus IL144 Nagashima, Sakiko; Kamimura, Akiko; Shimizu, Takayuki Journal of Bacteriology, Vol. 194, Issue 13 https://doi.org/10.1128/JB.00511-12	journal	July 2012
Draft Genome Sequence of Rubrivivax gelatinosus CBS Hu, P.; Lang, J.; Wawrousek, K. Journal of Bacteriology, Vol. 194, Issue 12 https://doi.org/10.1128/JB.00515-12	journal	May 2012
Sustaining N ₂ -Dependent Growth in the Presence of CO Kerby, Robert L.; Roberts, Gary P. Journal of Bacteriology, Vol. 193, Issue 3 https://doi.org/10.1128/JB.00794-10	journal	February 2011
Metabolism of carbon monoxide by Rhodopseudomonas gelatinosa: cell growth and properties of the oxidation system Uffen, R. L. Journal of Bacteriology, Vol. 155, Issue 3 https://doi.org/10.1128/JB.155.3.956-965.1983	journal	September 1983
Carbon monoxide dehydrogenase from Rhodospirillum rubrum Bonam, D.; Murrell, S. A.; Ludden, P. W. Journal of Bacteriology, Vol. 159, Issue 2 https://doi.org/10.1128/JB.159.2.693-699.1984	journal	August 1984
How Photosynthetic Bacteria Harvest Solar Energy Cogdell, Richard J.; Isaacs, Neil W.; Howard, Tina D. Journal of Bacteriology, Vol. 181, Issue 13 https://doi.org/10.1128/JB.181.13.3869-3879.1999	journal	July 1999
Ni ²⁺ Transport and Accumulation in Rhodospirillum rubrum Watt, Richard K.; Ludden, Paul W. Journal of Bacteriology, Vol. 181, Issue 15 https://doi.org/10.1128/JB.181.15.4554-4560.1999	journal	August 1999
Network of Hydrogenase Maturation in Escherichia coli : Role of Accessory Proteins HypA and HybF Hube, Michaela; Blokesch, Melanie; Böck, August Journal of Bacteriology, Vol. 184, Issue 14 https://doi.org/10.1128/JB.184.14.3879-3885.2002	journal	July 2002
Interaction between the H ₂ Sensor HupUV and the Histidine Kinase HupT Controls HupSL HydrogenaseSynthesis in Rhodobactercapsulatus Elsen, Sylvie; Duché, Ophélie; Colbeau, Annette Journal of Bacteriology, Vol. 185, Issue 24 https://doi.org/10.1128/JB.185.24.7111-7119.2003	journal	December 2003
[NiFe]-Hydrogenases of Ralstonia eutropha H16: Modular Enzymes for Oxygen-Tolerant Biological Hydrogen Oxidation Burgdorf, Tanja; Lenz, Oliver; Buhrke, Thorsten Journal of Molecular Microbiology and Biotechnology, Vol. 10, Issue 2-4 https://doi.org/10.1159/000091564	journal	January 2005
The RAST Server: Rapid Annotations using Subsystems Technology Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A. BMC Genomics, Vol. 9, Issue 1, Article No. 75 https://doi.org/10.1186/1471-2164-9-75	journal	January 2008

Cited By (1)

Transcriptome analysis of a thermophilic and hydrogenogenic carboxydotroph Carboxydothermus pertinax Fukuyama, Yuto; Omae, Kimiho; Yoshida, Takashi Extremophiles, Vol. 23, Issue 4 https://doi.org/10.1007/s00792-019-01091-x	journal	April 2019

Similar Records

Draft Genome Sequence of Rubrivivax gelatinosus CBS

Journal Article · Fri Jun 01 00:00:00 EDT 2012 · Journal of Bacteriology · OSTI ID:1050137

Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water–gas shift platform for H2 production

Journal Article · Sun Jun 30 20:00:00 EDT 2019 · Journal of Industrial Microbiology and Biotechnology · OSTI ID:1772501

Related Subjects

08 HYDROGEN
59 BASIC BIOLOGICAL SCIENCES
CO dehydrogenase
Hydrogenase
Rubrivivax gelatinosus
hydrogen
photosynthetic bacteria

Genome annotation provides insight into carbon monoxide and hydrogen metabolism in Rubrivivax gelatinosus

Citation Formats

References (76)

Cited By (1)

Similar Records

Related Subjects