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Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water–gas shift platform for H2 production

Journal Article · · Journal of Industrial Microbiology and Biotechnology
 [1];  [2];  [3];  [2];  [2];  [2]
  1. 0000 0001 2199 3636 grid.419357.d Biosciences Center National Renewable Energy Laboratory Golden CO USA, 0000000096214564 grid.266190.a Renewable and Sustainable Energy Institute University of Colorado Boulder CO USA
  2. 0000 0001 2199 3636 grid.419357.d Biosciences Center National Renewable Energy Laboratory Golden CO USA
  3. 0000 0001 2109 0381 grid.135963.b Chemical Engineering University of Wyoming Laramie WY USA
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Abstract

Biological H2 production has potential to address energy security and environmental concerns if produced from renewable or waste sources. The purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS produces H2 while oxidizing CO, a component of synthesis gas (Syngas). CO-linked H2 production is facilitated by an energy-converting hydrogenase (Ech), while a subsequent H2 oxidation reaction is catalyzed by a membrane-bound hydrogenase (MBH). Both hydrogenases contain [NiFe] active sites requiring 6 maturation factors (HypA-F) for assembly, but it is unclear which of the two annotated sets of hyp genes are required for each in R. gelatinosus CBS. Herein, we report correlated expression of hyp1 genes with Ech genes and hyp2 expression with MBH genes. Moreover, we find that while Ech H2 evolving activity is only delayed when hyp1 is deleted, hyp2 deletion completely disrupts MBH H2 uptake, providing a platform for a biologically driven water–gas shift reaction to produce H2 from CO.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Fuel Cell Technologies Program (EE-3F)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1772501
Alternate ID(s):
OSTI ID: 1508951
Report Number(s):
NREL/JA--2700-73765
Journal Information:
Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 7 Vol. 46; ISSN 1367-5435
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
Germany
Language:
English

References (35)

Duplication of hyp genes involved in maturation of [NiFe] hydrogenases in Alcaligenes eutrophus H16 journal November 1998
Hydrogen production from a carbon-monoxide oxidation pathway in Rubrivivax gelatinosus journal November 2002
A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants journal May 1998
Carboxydothermus hydrogenoformans gen. nov., sp. nov., a CO-utilizing Thermophilic Anaerobic Bacterium from Hydrothermal Environments of Kunashir Island journal July 1991
Maturation of the [NiFe] hydrogenases journal May 2001
CO-dependent H2 evolution by Rhodospirillum rubrum: Role of CODH:CooF complex journal December 2006
[NiFe]-Hydrogenase Maturation journal March 2016
Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview journal October 2007
Structure, function and biosynthesis of O2-tolerant hydrogenases journal January 2013
Recombinant and in vitro expression systems for hydrogenases: new frontiers in basic and applied studies for biological and synthetic H2 production journal January 2009
[NiFe]-Hydrogenase maturation endopeptidase: structure and function journal February 2005
Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans: CO-oxidizing:H2-evolving enzyme complex journal October 2002
Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation journal April 2002
Intact Functional Fourteen-subunit Respiratory Membrane-bound [NiFe]-Hydrogenase Complex of the Hyperthermophilic Archaeon Pyrococcus furiosus journal May 2014
HypF, a Carbamoyl Phosphate-converting Enzyme Involved in [NiFe] Hydrogenase Maturation journal October 2002
Concerted Action of Two Novel Auxiliary Proteins in Assembly of the Active Site in a Membrane-bound [NiFe] Hydrogenase journal November 2008
Taxonomy of the genus Cupriavidus: a tale of lost and found journal November 2004
Chaperones specific for the membrane-bound [NiFe]-hydrogenase interact with the Tat signal peptide of the small subunit precursor in Ralstonia eutropha H16 journal October 2007
hyp Gene Products in Alcaligenes Eutrophus are part of a Hydrogenase-Maturation System journal January 1996
Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants journal September 2005
Requirements for Construction of a Functional Hybrid Complex of Photosystem I and [NiFe]-Hydrogenase journal February 2010
Characterization of Genes Responsible for the CO-Linked Hydrogen Production Pathway in Rubrivivax gelatinosus journal April 2010
Characterization of the Oxygen Tolerance of a Hydrogenase Linked to a Carbon Monoxide Oxidation Pathway in Rubrivivax gelatinosus journal June 2002
Energy Generation from the CO Oxidation-Hydrogen Production Pathway in Rubrivivax gelatinosus journal June 2005
The Maturation Factors HoxR and HoxT Contribute to Oxygen Tolerance of Membrane-Bound [NiFe] Hydrogenase in Ralstonia eutropha H16 journal March 2011
Physiological function of hydrogen metabolism during growth of sulfidogenic bacteria on organic substrates. journal January 1984
Positive Transcriptional Feedback Controls Hydrogenase Expression in Alcaligenes eutrophusH16 journal September 1999
The H2 Sensor of Ralstonia eutropha Is a Member of the Subclass of Regulatory [NiFe] Hydrogenases journal May 2000
Involvement of hyp Gene Products in Maturation of the H2-Sensing [NiFe] Hydrogenase of Ralstonia eutropha journal December 2001
Requirements for Heterologous Production of a Complex Metalloenzyme: the Membrane-Bound [NiFe] Hydrogenase journal September 2005
Carbon monoxide-dependent growth of Rhodospirillum rubrum. journal April 1995
The Alcaligenes eutrophus membrane-bound hydrogenase gene locus encodes functions involved in maturation and electron transport coupling. journal August 1996
[NiFe]-Hydrogenases of Ralstonia eutropha H16: Modular Enzymes for Oxygen-Tolerant Biological Hydrogen Oxidation journal January 2005
Heterologous Expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] Hydrogenases in Synechococcus elongatus journal May 2011
Genome Annotation Provides Insight into Carbon Monoxide and Hydrogen Metabolism in Rubrivivax gelatinosus journal December 2014

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Related Subjects

08 HYDROGEN
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
CO oxidation
[NiFe] hydrogenase
hydrogenase maturation
water-gas shift reaction