Nitrogen regulation of lignin peroxidase gene transcription

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · Applied and Environmental Microbiology

OSTI ID:111393

Li, D; Alic, M; Gold, M H ^[1]

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1. Oregon Graduate Institute of Science and Technology, Portland, OR (United States)

Western blot (immunoblot) analysis with a polyclonal antibody to lignin peroxidase (LiP) isozyme H8 from the white rot basidiomycete Phanerochaete chrysosporium demonstrates that LiP protein is detectable in the extracellular media of 5- and 6-day-old nitrogen-limited, but not nitrogen-sufficient, cultures. Northern (RNA) blot analysis demonstrates that lip mRNA is detectable from 5- and 6-day old cells grown in nitrogen-limited, but not nitrogen-sufficient, cultures. These results indicate that LiP expression is regulated at the level of gene transcription by nutrient nitrogen. Since lignin degradation by P. chrysosporium is derepressed by nitrogen starvation, it appears that lignin degradation and LiP expression are coordinately regulated in this organism. These results contradict a recent report which concluded that LiP protein expression is not regulated by nutrient nitrogen. 28 refs., 2 figs.

• OSTI ID: 111393
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 9 Vol. 60; ISSN 0099-2240; ISSN AEMIDF
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL PATHWAYS
GENES
GENETIC CONTROL
LIGNIN
NITROGEN
PEROXIDASES
PHANEROCHAETE
RESPONSE MODIFYING FACTORS
TRANSCRIPTION