Mn(II) regulation of lignin peroxidases and manganese-dependent peroxidases from lignin-degrading white rot fungi

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6743917

Bonnarme, P; Jeffries, T W ^[1]

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1. Institute for Microbial and Biochemical Technology, Madison, WI (USA)

Two families of peroxidases-lignin peroxidase (LiP) and manganese-dependent lignin peroxidase (MnP)-are formed by the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium and other white rot fungi. Isoenzymes of these enzyme families carry out reactions important to the biodegradation of lignin. This research investigated the regulation of LiP and MnP production by Mn(II). In liquid culture, LiP titers varied as an inverse function of and MnP titers varied as a direct function of the Mn(II) concentration. The extracellular isoenzyme profiles differed radically at low and high Mn(II) levels, whereas other fermentation parameters, including extracellular protein concentrations, the glucose consumption rate, and the accumulation of cell dry weight, did not change significantly with the Mn(II) concentration. In the absence of Mn(II), extracellular LiP isoenzymes predominated, whereas in the presence of Mn(II), MnP isoenzymes were dominant. The release of {sup 14}CO{sub 2} from {sup 14}C-labeled dehydrogenative polymerizate lignin was likewise affected by Mn(II). The rate of {sup 14}CO{sub 2} release increased at low Mn(II) and decreased at high Mn(II) concentrations. This regulatory effect of Mn(II) occurred with five strains of P. chrysosporium, two other species of Phanerochaete, three species of Phlebia, Lentinula edodes, and Phellinus pini.

• OSTI ID: 6743917
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:1; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCONVERSION
BIODEGRADATION
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL REACTIONS
DECOMPOSITION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FERMENTATION
FUNGI
ISOENZYMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGNIN
MANGANESE
METALS
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
PLANTS
POLYSACCHARIDES
SACCHARIDES
TRACER TECHNIQUES
TRANSITION ELEMENTS