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Switchgrass ubiquitin promoter (PVUBI2) and uses thereof

Patent ·
OSTI ID:1109474
The subject application provides polynucleotides, compositions thereof and methods for regulating gene expression in a plant. Polynucleotides disclosed herein comprise novel sequences for a promoter isolated from Panicum virgatum (switchgrass) that initiates transcription of an operably linked nucleotide sequence. Thus, various embodiments of the invention comprise the nucleotide sequence of SEQ ID NO: 2 or fragments thereof comprising nucleotides 1 to 692 of SEQ ID NO: 2 that are capable of driving the expression of an operably linked nucleic acid sequence.
Research Organization:
ORNL (Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States))
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-00OR22725;
Assignee:
University of Tennessee Research Foundation (Knoxville, TN)
Patent Number(s):
8,604,276
Application Number:
12/797,248
OSTI ID:
1109474
Country of Publication:
United States
Language:
English

References (12)

Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselinum crispum journal February 1993
Activity of a maize ubiquitin promoter in transgenic rice journal November 1993
Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation journal February 1992
A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity journal January 1994
Structure and expression of sunflower ubiquitin genes journal September 1991
Characterization of a polyubiquitin gene from Arabidopsis thaliana journal August 1988
Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants journal May 1996
Structure and promoter activity of a stress and developmentally regulated polyubiquitin-encoding gene of Nicotiana tabacum journal October 1994
Comparative expression analysis of two sugarcane polyubiquitin promoters and flanking sequences in transgenic plants journal January 2003
PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences journal January 2002
Isolation of a Polyubiquitin Promoter and Its Expression in Transgenic Potato Plants journal December 1995
Comparative Genomics in Switchgrass Using 61,585 High-Quality Expressed Sequence Tags journal January 2008