Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Environmental stress-inducible promoter and its application in crops

Patent ·
OSTI ID:1531852

The subject application provides polynucleotides, compositions thereof and methods for regulating gene expression in a plant using a promoter that initiates transcription in an inducible manner. In a further aspect of the invention, methods for modulating expression of a gene product in a stably transformed plant comprising the steps of (a) transforming a plant cell with a DNA construct comprising the disclosed promoter or fragments thereof that are capable of driving the expression of an operably linked nucleic acid sequence operably linked to at least one nucleotide sequence; (b) growing the plant cell under plant growing conditions and (c) regenerating a stably transformed plant from the plant cell wherein the induced expression of the operably linked nucleotide sequence alters the phenotype of the plant.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-00OR22725
Assignee:
University of Tennessee Research Foundation (Knoxville, TN)
Patent Number(s):
8,471,100
Application Number:
12/770,943
OSTI ID:
1531852
Country of Publication:
United States
Language:
English

References (34)

Engineered GFP as a vital reporter in plants journal March 1996
Green fluorescent protein as a marker for gene expression journal February 1994
Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. journal January 1988
Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector journal May 1983
Plant salt tolerance journal February 2001
Luciferase reporter gene cassettes for plant gene expression studies journal January 1987
Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. journal August 1990
Map-based cloning of a protein kinase gene conferring disease resistance in tomato journal November 1993
Developing salt-tolerant crop plants: challenges and opportunities journal December 2005
Role of Cold-Responsive Genes in Plant Freezing Tolerance journal September 1998
Heat tolerance in plants: An overview journal December 2007
Development of submergence-tolerant rice cultivars: the Sub1 locus and beyond journal October 2008
Nucleotide sequence of barley chymotrypsin inhibitor-2 (CI-2) and its expression in normal and high-lysine barley journal May 1987
A Regulatory Gene as a Novel Visible Marker for Maize Transformation journal January 1990
Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice journal August 2006
Comparative functional analysis of three abiotic stress-inducible promoters in transgenic rice journal April 2009
Engineering herbicide resistance in plants by expression of a detoxifying enzyme journal September 1987
Herbicide Resistance in Transgenic Plants Expressing a Bacterial Detoxification Gene journal October 1988
Molecular marker survey and expression analyses of the rice submergence-tolerance gene SUB1A journal July 2010
Agronomic trait evaluation of field-grown transgenic rice plants containing the hygromycin resistance gene and the maize Activator element journal June 1995
Firefly luciferase gene: structure and expression in mammalian cells journal February 1987
Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters journal February 2005
Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa) journal November 2008
Rice DREB1B promoter shows distinct stress-specific responses, and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance journal August 2008
Chimeric genes as dominant selectable markers in plant cells journal June 1983
The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1 journal January 1986
Codon usage in plant genes journal January 1989
Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging journal November 1994
The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats journal September 1994
A marker-assisted backcross approach for developing submergence-tolerant rice cultivars journal July 2007
Signal transduction during cold, salt, and drought stresses in plants journal May 2011
Identifying novel QTLs for submergence tolerance in rice cultivars IR72 and Madabaru journal November 2011
Isolation and Functional Analysis of Arabidopsis Stress-Inducible NAC Transcription Factors That Bind to a Drought-Responsive cis -Element in the early responsive to dehydration stress 1 Promoter journal August 2004
[35] Transient expression analysis in plants using firefly luciferase reporter gene book January 1992

Similar Records

Switchgrass ubiquitin promoter (PVUBI2) and uses thereof
Patent · 2013 · OSTI ID:1109474

Nucleic acids encoding antifungal polypeptides and uses thereof
Patent · 2010 · OSTI ID:1014929

Antifungal polypeptides
Patent · 2007 · OSTI ID:921018