Microchannel gel electrophoretic separation systems and methods for preparing and using
Patent
·
OSTI ID:1093229
A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes, native PAGE conditions were employed. Both direct (non-competitive) and competitive immunoassay formats are demonstrated in microchips for detecting toxins and biomarkers (cytokines, c-reactive protein) in bodily fluids (serum, saliva, oral fluids). Further, a description of gradient gels fabrication is included, in an effort to describe methods we have developed for further optimization of on-chip PAGE immunoassays. The described chip-based PAGE immunoassay method enables immunoassays that are fast (minutes) and require very small amounts of sample (less than a few microliters). Use of microfabricated chips as a platform enables integration, parallel assays, automation and development of portable devices.
- Research Organization:
- SNL-A (Sandia National Laboratories, Albuquerque, NM (United States))
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC04-94AL85000
- Assignee:
- Sandia Corporation (Albuquerque, NM)
- Patent Number(s):
- 8,524,060
- Application Number:
- 12/728,797
- OSTI ID:
- 1093229
- Country of Publication:
- United States
- Language:
- English
Polyacrylamide gradient gel-filled capillaries with low detection background
|
journal | June 1997 |
On-Chip Native Gel Electrophoresis-Based Immunoassays for Tetanus Antibody and Toxin
|
journal | January 2005 |
Similar Records
Microchannel gel electrophoretic separation systems and methods for preparing and using
Electrophoretic device and method to separate and detect analyte ions
Protein microchips : use for immunoassay and enzymatic reactions.
Patent
·
Mon Feb 23 23:00:00 EST 2015
·
OSTI ID:1171095
Electrophoretic device and method to separate and detect analyte ions
Patent
·
Tue Jun 13 00:00:00 EDT 2023
·
OSTI ID:1998481
Protein microchips : use for immunoassay and enzymatic reactions.
Journal Article
·
Mon Feb 14 23:00:00 EST 2000
· Anal. Biochem.
·
OSTI ID:942617