Protein microchips : use for immunoassay and enzymatic reactions.
Different proteins such as antibodies, antigens, and enzymes were immobilized within the 100 x 100 x 20-{mu}m gel pads of protein microchips. A modified polyacrylamide gel has been developed to accommodate proteins of a size up to 400,000 daltons. Electrophoresis in the microchip reaction chamber speeded up antigen-antibody interactions within the gel. Protein microchips were used in immunoassays for detection of antigens or antibodies, as well as to carry out enzymatic reactions and to measure their kinetics in the absence or presence of an inhibitor. A protein microchip can be used several times in different immunoassays and enzymatic kinetic measurements.
- Research Organization:
- Argonne National Laboratory (ANL)
- Sponsoring Organization:
- DOD; FOR
- DOE Contract Number:
- AC02-06CH11357
- OSTI ID:
- 942617
- Report Number(s):
- ANL/BTC/JA-33080
- Journal Information:
- Anal. Biochem., Journal Name: Anal. Biochem. Journal Issue: 2 ; Feb. 15, 2000 Vol. 278; ISSN ANBCA2; ISSN 0003-2697
- Country of Publication:
- United States
- Language:
- ENGLISH
Similar Records
Immobilization of DNA in polyacrylamide gel for the manufacture of DNA and DNA-oligonucleotide microchips.
Massive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips.
Microchannel gel electrophoretic separation systems and methods for preparing and using
Journal Article
·
Fri May 15 00:00:00 EDT 1998
· Anal. Biochem.
·
OSTI ID:938359
Massive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips.
Journal Article
·
Fri Jun 15 00:00:00 EDT 2001
· Nucleic Acids Res.
·
OSTI ID:942988
Microchannel gel electrophoretic separation systems and methods for preparing and using
Patent
·
Mon Feb 23 23:00:00 EST 2015
·
OSTI ID:1171095