Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them

Gray, Kevin A; Zhao, Lishan; Cayouette, Michelle H

Title: Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them

Patent · Tue Jan 24 00:00:00 EST 2012

OSTI ID:1034636

Gray, Kevin A ^[1]; Zhao, Lishan ^[2]; Cayouette, Michelle H ^[1]

San Diego, CA
Emeryville, CA

The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a .beta.-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, .beta.-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

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Research Organization:: EI DUPORN NEMOURS & CO

Sponsoring Organization:: USDOE

DOE Contract Number:: FC36-03GO13146

Assignee:: BP Corporation North America Inc. (Houston, TX)

Patent Number(s):: 8,101,393

Application Number:: 12/278,958

OSTI ID:: 1034636

Country of Publication:: United States

Language:: English

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