skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion"

Abstract

We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
DOE - OFFICE OF SCIENCE
OSTI Identifier:
1034084
Report Number(s):
BNL-93649-2011-JA
Journal ID: ISSN 1936-0851; R&D Project: MA-114-MAEA; KC0203010; TRN: US201203%%384
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Journal Name:
ACS Nano
Additional Journal Information:
Journal Volume: 5; Journal Issue: 4; Journal ID: ISSN 1936-0851
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; DNA; FABRICATION; FASTENING; GOLD; HYBRIDIZATION; NUCLEIC ACIDS; PEPTIDES; SPECIFICITY; gold nanoparticles; peptide nucleic acid; double-stranded DNA; nanoparticle assembly

Citation Formats

Stadler, A L, van der Lelie, D, Sun, D, Maye, M M, and Gang, O. Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion". United States: N. p., 2011. Web. doi:10.1021/nn101355n.
Stadler, A L, van der Lelie, D, Sun, D, Maye, M M, & Gang, O. Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion". United States. doi:10.1021/nn101355n.
Stadler, A L, van der Lelie, D, Sun, D, Maye, M M, and Gang, O. Fri . "Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion"". United States. doi:10.1021/nn101355n.
@article{osti_1034084,
title = {Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion"},
author = {Stadler, A L and van der Lelie, D and Sun, D and Maye, M M and Gang, O},
abstractNote = {We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.},
doi = {10.1021/nn101355n},
journal = {ACS Nano},
issn = {1936-0851},
number = 4,
volume = 5,
place = {United States},
year = {2011},
month = {4}
}