Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion"

Journal Article · Fri Apr 01 00:00:00 EDT 2011 · ACS Nano

DOI:https://doi.org/10.1021/nn101355n· OSTI ID:1034084

Stadler, A L; van der Lelie, D; Sun, D; Maye, M M; Gang, O

We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

• Research Organization: Brookhaven National Lab. (BNL), Upton, NY (United States)
• Sponsoring Organization: DOE - OFFICE OF SCIENCE
• DOE Contract Number: DE-AC02-98CH10886
• OSTI ID: 1034084
• Report Number(s): BNL-93649-2011-JA; R&D Project: MA-114-MAEA; KC0203010; TRN: US201203%%384
• Journal Information: ACS Nano, Vol. 5, Issue 4; ISSN 1936-0851
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
DNA
FABRICATION
FASTENING
GOLD
HYBRIDIZATION
NUCLEIC ACIDS
PEPTIDES
SPECIFICITY
gold nanoparticles
peptide nucleic acid
double-stranded DNA
nanoparticle assembly