Double stranded nucleic acid biochips

Chernov, Boris; Golova, Julia

Double stranded nucleic acid biochips

Patent · Tue May 23 04:00:00 EDT 2006

OSTI ID:1175749

Chernov, Boris; Golova, Julia

This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.

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Research Organization:: The University Of Chicago, Chicago, IL (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-31109-ENG-38

Assignee:: The University of Chicago (Chicago, IL)

Patent Number(s):: 7,049,073

Application Number:: 10/283,670

OSTI ID:: 1175749

Country of Publication:: United States

Language:: English

References (16)

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