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Title: High expression Zymomonas promoters

Patent ·
OSTI ID:1026671
 [1];  [2];  [3];  [4];  [5];  [6];  [7]
  1. West Chester, PA
  2. Havertown, PA
  3. New Hope, PA
  4. Kennett Square, PA
  5. Lakewood, CO
  6. Wilmington, DE
  7. Centennial, CO
+ Show Author Affiliations

Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.

Research Organization:
E.I. du Pont de Nemours and Company Alliance for Sustainable Energy LLC (Wilmington, DE)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-03GO13146; AC36-99GO10337
Assignee:
E.I. du Pont de Nemours and Company Alliance for Sustainable Energy LLC (Wilmington, DE)
Patent Number(s):
7,989,206
Application Number:
12/410,495
OSTI ID:
1026671
Country of Publication:
United States
Language:
English

References (8)

Genetic Engineering of Zymobacter palmae for Production of Ethanol from Xylose journal February 2007
Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains journal December 1992
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis journal January 1995
Sequences of versatile broad-host-range vectors of the RK2 family journal July 2003
Promoter selectivity of Escherichia coli RNA polymerase: effect of base substitutions in the promoter −35 region on promoter strength journal January 1990
An efficient system for markerless gene replacement applicable in a wide variety of enterobacterial species journal January 2007
Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli journal January 2006
Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content journal August 2003

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Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing, and identification of promoter region
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Related Subjects

59 BASIC BIOLOGICAL SCIENCES