Phosphoglycerate kinase gene from Zymomonas mobilis: cloning sequencing, and localization within the gap operon
The Zymomonas mobilis gene encoding phosphoglycerate kinase (EC 2.7.3.2), pgk, has been cloned into Escherichia coli and sequenced. It consists of 336 amino acids, including the N-terminal methionine, with a molecular weight of 47,384. This promoterless gene is located 225 base pairs downstream from the gap gene and is part of the gap operon. Previous studies have shown that the specific activities of glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase do not change coordinately in Z. mobilis, although the two enzymes appear to be under the control of a common promoter. The translated amino acid sequence for the Z. mobilis phosphoglycerte kinase is less conserved than those of eucaryotic genes. A comparison of known sequences for phosphoglycerate kinase revealed a high degree of conservation of structure with 102 amino acid positions being retained by all. In general, the amino acid positions at the boundaries of ..beta..-sheet and ..cap alpha..-helical regions and those connecting these regions were more highly conserved than the amino acid positions within regions of secondary structure.
- Research Organization:
- Univ. of Florida, Gainesville (USA)
- DOE Contract Number:
- FG05-86ER13575
- OSTI ID:
- 6998606
- Journal Information:
- J. Bacteriol.; (United States), Vol. 170:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PHOSPHOTRANSFERASES
DNA SEQUENCING
DNA-CLONING
GENETIC MAPPING
ZYMOMONAS MOBILIS
ENZYME ACTIVITY
AMINO ACID SEQUENCE
ESCHERICHIA COLI
GENE OPERONS
GENE REPRESSORS
RECOMBINANT DNA
BACTERIA
CLONING
DNA
ENZYMES
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550200* - Biochemistry
550400 - Genetics