Phosphoglycerate kinase gene from Zymomonas mobilis: cloning sequencing, and localization within the gap operon

Journal Article · Fri Apr 01 04:00:00 EST 1988 · J. Bacteriol.; (United States)

OSTI ID:6998606

Conway, T; Ingram, L O

The Zymomonas mobilis gene encoding phosphoglycerate kinase (EC 2.7.3.2), pgk, has been cloned into Escherichia coli and sequenced. It consists of 336 amino acids, including the N-terminal methionine, with a molecular weight of 47,384. This promoterless gene is located 225 base pairs downstream from the gap gene and is part of the gap operon. Previous studies have shown that the specific activities of glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase do not change coordinately in Z. mobilis, although the two enzymes appear to be under the control of a common promoter. The translated amino acid sequence for the Z. mobilis phosphoglycerte kinase is less conserved than those of eucaryotic genes. A comparison of known sequences for phosphoglycerate kinase revealed a high degree of conservation of structure with 102 amino acid positions being retained by all. In general, the amino acid positions at the boundaries of ..beta..-sheet and ..cap alpha..-helical regions and those connecting these regions were more highly conserved than the amino acid positions within regions of secondary structure.

• Research Organization: Univ. of Florida, Gainesville (USA)
• DOE Contract Number: FG05-86ER13575
• OSTI ID: 6998606
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 170:4; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BACTERIA
CLONING
DNA
DNA SEQUENCING
DNA-CLONING
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
GENE OPERONS
GENE REPRESSORS
GENETIC MAPPING
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
ZYMOMONAS MOBILIS