Zymomonas with improved xylose utilization

Viitanen, Paul V; Tao, Luan; Zhang, Yuying; Caimi, Perry G; McCutchen, Carol M; McCole, Laura; Zhang, Min; Chou, Yat-Chen; Franden, Mary Ann

Zymomonas with improved xylose utilization

Patent · Tue Aug 16 00:00:00 EDT 2011

OSTI ID:1025535

Viitanen, Paul V ^[1]; Tao, Luan ^[2]; Zhang, Yuying ^[3]; Caimi, Perry G ^[4]; McCutchen, Carol M ^[5]; McCole, Laura ^[6]; Zhang, Min ^[7]; Chou, Yat-Chen ^[7]; Franden, Mary Ann ^[8]

West Chester, PA
Havertown, PA
New Hope, PA
Kennett Square, PA
Wilmington, DE
East Fallowfield, PA
Lakewood, CO
Centennial, CO

Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

View Patent

Research Organization:: E. I. du Pont de Nemours and Company (Wilmington, DE)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC36-03GO13146

Assignee:: E. I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (Wilmington, DE)

Patent Number(s):: 7,998,722

Application Number:: 12/410,501

OSTI ID:: 1025535

Country of Publication:: United States

Language:: English

References (24)

Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains Feldmann, SigrunD.; Sahm, Hermann; Sprenger, GeorgA. Applied Microbiology and Biotechnology, Vol. 38, Issue 3 https://doi.org/10.1007/BF00170086	journal	December 1992
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis Zhang, M.; Eddy, C.; Deanda, K. Science, Vol. 267, Issue 5195 https://doi.org/10.1126/science.267.5195.240	journal	January 1995
Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content Cahoon, Edgar B.; Hall, Sarah E.; Ripp, Kevin G. Nature Biotechnology, Vol. 21, Issue 9 https://doi.org/10.1038/nbt853	journal	August 2003
Fast and sensitive multiple sequence alignments on a microcomputer Higgins, Desmond G.; Sharp, Paul M. Bioinformatics, Vol. 5, Issue 2, p. 151-153 https://doi.org/10.1093/bioinformatics/5.2.151	journal	April 1989
Testing statistical significance scores of sequence comparison methods with structure similarity Hulsen, Tim; de Vlieg, Jacob; Leunissen, Jack AM BMC Bioinformatics, Vol. 7, Issue 1 https://doi.org/10.1186/1471-2105-7-444	journal	October 2006
Pfam: clans, web tools and services Finn, R. D. Nucleic Acids Research, Vol. 34, Issue 90001 https://doi.org/10.1093/nar/gkj149	journal	January 2006
Biological Sequence Analysis Durbin, Richard; Eddy, Sean R.; Krogh, Anders Cambridge University Press https://doi.org/10.1017/CBO9780511790492	book	January 2012
Computer Analysis of Sequence Data, Part I Griffin, Annette M.; Griffin, Hugh G. https://doi.org/10.1385/0896032469	book	February 1994
The use of oliodeoxynucleotide probes in chaotrope-based hybridization solutions Ness, Jeffery Van; Chen, Lennie Nucleic Acids Research, Vol. 19, Issue 19 https://doi.org/10.1093/nar/19.19.5143	journal	January 1991
Sequence Analysis Primer Gribskov, Michael; Devereux, John https://doi.org/10.1007/978-1-349-21355-9	book	January 1991
CLUSTAL V: improved software for multiple sequence alignment Higgins, Desmond G.; Bleasby, Alan J.; Fuchs, Rainer Bioinformatics, Vol. 8, Issue 2 https://doi.org/10.1093/bioinformatics/8.2.189	journal	January 1992
Sequences of versatile broad-host-range vectors of the RK2 family Scott, Heather N.; Laible, Philip D.; Hanson, Deborah K. Plasmid, Vol. 50, Issue 1, p. 74-79 https://doi.org/10.1016/S0147-619X(03)00030-1	journal	July 2003
Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant Deshpande, Mukund V. Applied Biochemistry and Biotechnology, Vol. 36, Issue 3, p. 227-234 https://doi.org/10.1007/BF02921781	journal	September 1992
Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain Loh, E.; Elliott, J.; Cwirla, S. Science, Vol. 243, Issue 4888 https://doi.org/10.1126/science.2463672	journal	January 1989
Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing, and identification of promoter region. Conway, T.; Sewell, G. W.; Ingram, L. O. Journal of Bacteriology, Vol. 169, Issue 12 https://doi.org/10.1128/JB.169.12.5653-5662.1987	journal	December 1987
Genetic Engineering of Zymobacter palmae for Production of Ethanol from Xylose Yanase, H.; Sato, D.; Yamamoto, K. Applied and Environmental Microbiology, Vol. 73, Issue 8 https://doi.org/10.1128/AEM.02302-06	journal	February 2007
Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Walker, G. T.; Little, M. C.; Nadeau, J. G. Proceedings of the National Academy of Sciences, Vol. 89, Issue 1, p. 392-396 https://doi.org/10.1073/pnas.89.1.392	journal	January 1992
Hidden Markov Models in Computational Biology Krogh, Anders; Brown, Michael; Mian, I. Saira Journal of Molecular Biology, Vol. 235, Issue 5 https://doi.org/10.1006/jmbi.1994.1104	journal	February 1994
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002
Searching Protein Sequence Databases — Is Optimal Best? Pearson, William R. Computational Methods in Genome Research https://doi.org/10.1007/978-1-4615-2451-9_9	book	January 1994
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Tabor, S.; Richardson, C. C. Proceedings of the National Academy of Sciences, Vol. 82, Issue 4 https://doi.org/10.1073/pnas.82.4.1074	journal	February 1985
Cloning and expression of the genes for xylose isomerase and xylulokinase from Klebsiella pneumoniae 1033 in Escherichia coli K12 Feldmann, Sigrun D.; Sahm, Hermann; Sprenger, Georg A. Molecular and General Genetics MGG, Vol. 234, Issue 2 https://doi.org/10.1007/BF00283840	journal	August 1992
One-sided polymerase chain reaction: the amplification of cDNA. Ohara, O.; Dorit, R. L.; Gilbert, W. Proceedings of the National Academy of Sciences, Vol. 86, Issue 15 https://doi.org/10.1073/pnas.86.15.5673	journal	August 1989
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990

Similar Records

Single zymomonas mobilis strain for xylose and arabinose fermentation

Patent · Wed Dec 31 23:00:00 EST 1997 · OSTI ID:872008

Recombinant Zymomonas mobilis with improved xylose utilization

Patent · Tue May 20 00:00:00 EDT 2003 · OSTI ID:1007472

Single Zymomonas mobilis strain for xylose and arabinose fermentation

Patent · Mon Nov 30 23:00:00 EST 1998 · OSTI ID:321178

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Zymomonas with improved xylose utilization

Citation Formats

References (24)

Similar Records

Related Subjects