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Title: Single zymomonas mobilis strain for xylose and arabinose fermentation

Patent ·
OSTI ID:872008
 [1];  [2];  [3];  [4]
  1. Lakewood, CO
  2. Wheat Ridge, CO
  3. Landenberg, PA
  4. Fort Collins, CO
+ Show Author Affiliations

This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.

Research Organization:
Midwest Research Institute, Kansas City, MO (United States)
DOE Contract Number:
AC36-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MI)
Patent Number(s):
US 5843760
OSTI ID:
872008
Country of Publication:
United States
Language:
English

References (4)

The organization of the araBAD operon of Escherichia coli journal January 1986
Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains journal December 1992
Approaches to broaden the substrate and product range of the ethanologenic bacterium Zymomonas mobilis by genetic engineering journal February 1993
Molecular characterization of the Zymomonas mobilis enolase (eno) gene. journal January 1992

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Related Subjects

single
zymomonas
mobilis
strain
xylose
arabinose
fermentation
relates
microorganisms
normally
ferment
pentose
sugars
genetically
altered
produce
ethanol
process
utilizing
examples
transformed
combination
coli
genes
isomerase
xylulokinase
l-arabinose
l-ribulokinase
l-ribulose
5-phosphate
4-epimerase
transaldolase
transketolase
expression
added
control
z
promoters
newly
created
useful
fermenting
glucose
produced
hydrolysis
hemicellulose
cellulose
starch
xylose isomerase
pentose sugar
fermentation process
zymomonas mobilis
genetically altered
produce ethanol
process utilizing
coli genes
newly created
mobilis promoters
ferment pentose
added genes
pentose sugars
created microorganisms
l-arabinose isomerase
fermenting glucose
/435/999/