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Single zymomonas mobilis strain for xylose and arabinose fermentation

Patent ·
OSTI ID:872008
 [1];  [2];  [3];  [4]
  1. Lakewood, CO
  2. Wheat Ridge, CO
  3. Landenberg, PA
  4. Fort Collins, CO
+ Show Author Affiliations

This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.

Research Organization:
Midwest Research Institute
DOE Contract Number:
AC36-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MI)
Patent Number(s):
US 5843760
OSTI ID:
872008
Country of Publication:
United States
Language:
English

References (4)

Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains journal December 1992
The organization of the araBAD operon of Escherichia coli journal January 1986
Molecular characterization of the Zymomonas mobilis enolase (eno) gene. journal January 1992
Approaches to broaden the substrate and product range of the ethanologenic bacterium Zymomonas mobilis by genetic engineering journal February 1993

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Related Subjects

/435/999/
4-epimerase
5-phosphate
added
added genes
altered
arabinose
cellulose
coli
coli genes
combination
control
created
created microorganisms
ethanol
examples
expression
ferment
ferment pentose
fermentation
fermentation process
fermenting
fermenting glucose
genes
genetically
genetically altered
glucose
hemicellulose
hydrolysis
isomerase
l-arabinose
l-arabinose isomerase
l-ribulokinase
l-ribulose
microorganisms
mobilis
mobilis promoters
newly
newly created
normally
pentose
pentose sugar
pentose sugars
process
process utilizing
produce
produce ethanol
produced
promoters
relates
single
starch
strain
sugars
transaldolase
transformed
transketolase
useful
utilizing
xylose
xylose isomerase
xylulokinase
z
zymomonas
zymomonas mobilis