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Pentose fermentation by recombinant zymomonas

Patent ·
OSTI ID:871344
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [8]
  1. Golden, CO
  2. Lakewood, CO
  3. Saratoga Springs, NY
  4. Conifer, CO
  5. Fort Collins, CO
  6. Northglenn, CO
  7. Littleton, CO
  8. Boulder, CO
+ Show Author Affiliations

The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose 5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.

Research Organization:
Midwest Research Institute
DOE Contract Number:
AC36-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Number(s):
US 5712133
OSTI ID:
871344
Country of Publication:
United States
Language:
English

References (6)

Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing, and identification of promoter region. journal December 1987
Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains journal December 1992
Expression of cloned Xanthomonasd-xylose catabolic genes in Zymomonas mobilis journal January 1988
Systematic sequencing of the Escherichia coli genome: analysis of the 0 – 2.4 min region journal January 1992
Molecular characterization of the Zymomonas mobilis enolase (eno) gene. journal January 1992
Approaches to broaden the substrate and product range of the ethanologenic bacterium Zymomonas mobilis by genetic engineering journal February 1993

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Related Subjects

/435/
4-epimerase
5-phosphate
added
added genes
altered
cellulose
coli
coli genes
combinations
control
created
created microorganisms
ethanol
examples
expression
ferment
ferment pentose
fermentation
fermentation process
fermentation processes
fermenting
fermenting pentoses
genes
genetically
genetically altered
glucose
hemicellulose
hydrolysis
isomerase
l-arabinose
l-arabinose isomerase
l-ribulokinase
l-ribulose
microorganisms
mobilis
mobilis promoters
newly
newly created
normally
pentose
pentose fermentation
pentose sugar
pentoses
processes
processes utilizing
produce
produce ethanol
produced
promoters
recombinant
recombinant zymomonas
relates
sugar
transaldolase
transformed
transketolase
useful
utilizing
xylose
xylose isomerase
xylulokinase
zymomonas
zymomonas mobilis