Correlation of chromosome patterns in human leukemic cells with exposure to chemicals and/or radiation. Comprehensive progress report, July 1991--June 1994

Technical Report · Wed Jun 01 04:00:00 EDT 1994

DOI:https://doi.org/10.2172/10162456· OSTI ID:10162456

Rowley, J D

This comprehensive progress report provides a synopsis of major research accomplishments during the years of 1991-1994, including the technical aspects of the project. The objectives and accomplishments are as follows: 1. Defining the chromosome segments associated with radiation and chemically-induced leukemogenesis (treatment-related acute myeloid leukemia, t-AML); A. Continued genetic analysis of chromosomes 5 and 7, B. Correlation of treatment with balanced and unbalanced translocations. 2. Cloning the breakpoints in balanced translocations in t-AML; A. Clone the t(9;11) and t(11;19) breakpoints, B. Clone the t(3,21)(q26,q22) breakpoint, C. Determine the relationship of these translocations to prior exposure to topoisomerase II inhibitors. 3. Compare the breakpoint junctions in patients who have the same translocations in t-AML and AML de novo. 4. Map the scaffold attachment regions in the genes that are involved in balanced translocations in t-AML. Plans for the continuation of present objectives and possible new objectives in consideration of past results are also provided.

• Research Organization: Chicago Univ., IL (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-86ER60408
• OSTI ID: 10162456
• Report Number(s): DOE/ER/60408--6; ON: DE94014506
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
CHEMOTHERAPY
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
CHROMOSOMES
CLONING
CYTOLOGY
GENETIC EFFECTS
GENETIC MAPPING
GENETIC RADIATION EFFECTS
GENETICS
HUMAN CHROMOSOME 5
HUMAN CHROMOSOME 7
LEUKEMOGENESIS
MYELOID LEUKEMIA
PATHOLOGY
PROGRESS REPORT
STRAND BREAKS