An investigation of liquid secondary ion and laser desorption mass spectroscopy for the analysis of planar chromatograms

Thesis/Dissertation · Thu Nov 01 04:00:00 EST 1990

OSTI ID:10144008

Dunphy, J C

In the work described in this dissertation, interfaces between two mass spectrometric methods, liquid secondary ion mass spectrometry (LSIMS) and laser desorption/ionization Fourier transform mass spectrometry (LD/FTMS), and thin-layer chromatography (TLC) and slab gel electrophoresis were developed for bioanalytical applications. In an investigation of direct LSIMS for TLC analysis (TLC/LSIMS), mass spectra of bile acids and bile salts were characterized directly from high-performance TLC plates. The scanning ability of the LSIMS instrument was used to generate spatial profiles of the characteristic bile acid ions in the mass spectra. A procedure for the analysis of bile salts in dog bile was developed involving an extraction step, followed by TLC separation and direct TLC/LSIMS detection and semi-quantitation. For peptides, an experiment called ``selected-sequence monitoring`` was developed to locate target peptides related in structure in complex mixtures developed on TLC plates. Ions characteristic of the bradykinin and enkephalin peptides were used to generate spatial profiles of members of those peptide families on TLC plates. Using a Fourier transform mass spectrometer (FTMS), a fundamental investigation was conducted into the factors affecting the quality of analytical data obtained using direct laser desorption/ionization to produce mass spectra from TLC plates.

• Research Organization: Indiana Univ., Bloomington, IN (United States). Dept. of Chemistry
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC05-76OR00033
• OSTI ID: 10144008
• Report Number(s): DOE/OR/00033--T475; ON: DE92013655
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102
400105
550200
59 BASIC BIOLOGICAL SCIENCES
BILE ACIDS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BRADYKININ
CHEMICAL ANALYSIS
CHEMICAL AND SPECTRAL PROCEDURES
DESORPTION
ELECTROPHORESIS
ENKEPHALINS
FOURIER TRANSFORM SPECTROMETERS
GELS
MASS SPECTROSCOPY
OLIGONUCLEOTIDES
POLYAMIDES
SEPARATION PROCEDURES
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY