Applications and current limitations of matrix-assisted laser desorption Fourier transform mass spectrometry for biomolecules
The recent advances in matrix-assisted laser desorption mass spectrometry have indicated tremendous potential for the analysis of large biomolecules (MW > 100,000 Daltons) such as peptides and oligonucleotides. To achieve soft ionization for these large molecules, a matrix compound such as nicotinic acid is mixed with a small amount of the biological analyte. This matrix helps absorb the laser radiation and subsequently reduce the fragmentation of the analyte ions. The objective of our research is to develop and refine laser desorption Fourier Transform Mass Spectrometry (FTMS) for the detailed structural characterization of biomolecules at the isomeric level. This presentation will focus on the development and application of matrix-assisted laser desorption FTMS for the examination of normal and modified oligonucleotides. Current research involves investigation and optimization of experimental parameters which will extend the mass range for the FTMS experiments. 4 refs., 1 fig.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 6223678
- Report Number(s):
- CONF-9104192-1; ON: DE91008877
- Resource Relation:
- Conference: Workshop on laser ablation: mechanisms and application, Oak Ridge, TN (USA), 8-10 Apr 1991
- Country of Publication:
- United States
- Language:
- English
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ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
OLIGONUCLEOTIDES
MASS SPECTRA
MOLECULAR STRUCTURE
POLYPEPTIDES
FOURIER TRANSFORM SPECTROMETERS
ION-MOLECULE COLLISIONS
IONIZATION
LASER RADIATION
NEAR ULTRAVIOLET RADIATION
NICOTINIC ACID
AZINES
CARBOXYLIC ACIDS
COLLISIONS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ION COLLISIONS
MEASURING INSTRUMENTS
MOLECULE COLLISIONS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PROTEINS
PYRIDINES
RADIATIONS
SPECTRA
SPECTROMETERS
ULTRAVIOLET RADIATION
VITAMIN B GROUP
VITAMINS
400102* - Chemical & Spectral Procedures
550200 - Biochemistry