Matrix-assisted laser desorption fourier transform mass spectrometry for biological compounds

Conference · Mon Jan 01 00:00:00 EST 1990

OSTI ID:6547571

Hettich, R; Buchanan, M

The recent development of matrix-assisted UV laser desorption (LD) mass spectrometry has made possible the ionization and detection of extremely large molecules (with molecular weights exceeding 100,000 Daltons). This technique has generated enormous interest in the biological community for the direct examination of large peptides and oligonucleotides. Although this matrix-assisted ionization method has been developed and used almost exclusively with time-of-flight (TOF) mass spectrometers, research is currently in progress to demonstrate this technique with trapped ion mass spectrometers, such as Fourier transform ion cyclotron resonance mass spectrometry (FTMS). The potential capabilities of FTMS for wide mass range, high resolution measurement, and ion trapping experiments suggest that this instrumental technique should be useful for the detailed structural characterization of large ions generated by the matrix-assisted technique. We have recently demonstrated that matrix-assisted ultraviolet laser desorption can be successfully used with FTMS for the ionization of small peptides. The objective of this report is to summarize the application and current limitations of matrix-assisted laser desorption FTMS for the characterization of peptides and oligonucleotides at the isomeric level. 4 refs., 3 figs., 2 tabs.

• Research Organization: Oak Ridge National Lab., TN (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6547571
• Report Number(s): CONF-9006271-2; ON: DE91000393
• Resource Relation: Conference: NATO advanced research workshop on methods and mechanisms for producing ions from large molecules, Ontario (Canada), 24-28 Jun 1990
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
OLIGONUCLEOTIDES
MASS SPECTROSCOPY
PEPTIDES
BIOLOGICAL MATERIALS
ENKEPHALINS
FOURIER TRANSFORM SPECTROMETERS
INSULIN
LASERS
MATRIX MATERIALS
MOLECULAR IONS
NICOTINIC ACID
ULTRAVIOLET SPECTROMETERS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
CARBOXYLIC ACIDS
CHARGED PARTICLES
DRUGS
ENDORPHINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
IONS
MATERIALS
MEASURING INSTRUMENTS
NEUROREGULATORS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
POLYPEPTIDES
PROTEINS
PYRIDINES
SPECTROMETERS
SPECTROSCOPY
VITAMIN B GROUP
VITAMINS
400102* - Chemical & Spectral Procedures