Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex

Journal Article · Mon Jul 28 04:00:00 EDT 2008 · Acta Crystallogr. F

DOI:https://doi.org/10.1107/S1744309108019404· OSTI ID:1006714

Hansen, Guido; Hercus, Timothy R; Xu, Yibin; Lopez, Angel F; Parker, Michael W; McKinstry, William J ^[1]

---

1. SVIMR-A

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts through a ternary receptor signalling complex containing specific {alpha} (GMR{alpha}) and common {beta} ({beta}c) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMR{alpha} and {beta}c are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMR{alpha} subunit and either {beta}c or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the {beta}c subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6{sub 3}22 and diffracted to 3.3 {angstrom} resolution.

• Research Organization: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
• Sponsoring Organization: USDOE
• OSTI ID: 1006714
• Journal Information: Acta Crystallogr. F, Journal Name: Acta Crystallogr. F Journal Issue: (8) ; 08, 2008 Vol. 64; ISSN 1744-3091
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ASTHMA
CRYSTALLIZATION
GENES
GROWTH FACTORS
LEUKEMIA
LYMPHOKINES
MUTANTS
MUTATIONS
POLYETHYLENE GLYCOLS
PROLINE
RECEPTORS
RESOLUTION
RHEUMATIC DISEASES
SPACE GROUPS
X-RAY DIFFRACTION