Gene for the [alpha]-subunit of the human interleukin-3 receptor (IL3RA) localized to the X-Y pseudoautosomal region
- Stanford Univ. School of Medicine, CA (United States)
- DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA (United States)
Interleukin-3 (IL3) and granulocyte/macrophage colony-stimulating factor (CSF2) stimulate proliferation and differentiation of various hemopoietic cell types. As is characteristic of the cytokine receptor family, the receptors for these proteins comprise [alpha]- and [beta]-subunits. While IL3 and CSF2 receptors each have unique [alpha]-subunits, they share a common [beta]-subunit. By Southern analysis of somatic cell hybrid panels, pulsed-field gel electrophoresis (PFGE), and fluorescence chromosomal in situ hybridization, the authors have mapped the cloned sequence for the IL3 receptor [alpha] (IL3RA) to the X-Y pseudoautosomal region at bands Xp22.3 and Yp11.3, near the gene for the [alpha]-subunit of the CSF2 receptor (CSF2RA). The CSF2RA and IL3RA genes are so close that their order could not be determined by two-color interphase in situ hybridization. They share PFGE fragments generated by different restriction enzymes down to the 50-100-kb size range. Pseudoautosomal inheritance was demonstrated by an EcoRI RFLP detected with the IL3RA cDNA probe. 47 refs., 6 figs.
- OSTI ID:
- 5428533
- Journal Information:
- American Journal of Human Genetics; (United States), Journal Name: American Journal of Human Genetics; (United States) Vol. 53:5; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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