Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex
- Biota Structural Biology Laboratory, St Vincent’s Institute of Medical Research, 41 Victoria Parade, Fitzroy, Victoria 3065 (Australia)
- Cytokine Receptor Laboratory, Division of Human Immunology, Institute of Medical and Veterinary Science (IMVS), Frome Road, Adelaide, South Australia 5001 (Australia)
Crystals of the soluble ternary GM-CSF receptor complex were obtained which diffracted to a resolution of 3.3 Å. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts though a ternary receptor signalling complex containing specific α (GMRα) and common β (βc) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMRα and βc are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMRα subunit and either βc or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the βc subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6{sub 3}22 and diffracted to 3.3 Å resolution.
- OSTI ID:
- 22360605
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 8 Vol. 64; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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