Title: DNA polymerase having modified nucleotide binding site for DNA sequencing

Abstract

Modified gene encoding a modified DNA polymerase wherein the modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase.

Inventors:

Tabor, Stanley ^[1]; Richardson, Charles ^[2]

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1. Cambridge, MA
2. Chestnut Hill, MA

Issue Date:

Wed Jan 01 00:00:00 EST 1997

Research Org.:

Harvard University

OSTI Identifier:

870881

Patent Number(s):

5614365

Assignee:

President & Fellow of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/1252 - {DNA-directed DNA polymerase

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/686 - Polymerase chain reaction [PCR]
C12Q1/6869 - Methods for sequencing

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DOE Contract Number:  

FG02-88ER60688

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

dna; polymerase; modified; nucleotide; binding; site; sequencing; encoding; incorporates; dideoxynucleotides; 20-fold; compared; corresponding; deoxynucleotides; naturally-occurring; dna polymerase; binding site; dna sequencing; modified polymer; /435/530/999/