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Title: DNA polymerase with modified processivity

Abstract

Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

Inventors:
 [1];  [2];  [3]
  1. Brookline, MA
  2. Cambridge, MA
  3. Chestnut Hill, MA
Issue Date:
Research Org.:
Harvard University
OSTI Identifier:
872606
Patent Number(s):
5972603
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-88ER60688
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
dna; polymerase; modified; processivity; chimeric; domain; factor; binding; naturally; associated; binding domain; dna polymerase; polymerase domain; /435/530/

Citation Formats

Bedford, Ella, Tabor, Stanley, and Richardson, Charles C. DNA polymerase with modified processivity. United States: N. p., 1999. Web.
Bedford, Ella, Tabor, Stanley, & Richardson, Charles C. DNA polymerase with modified processivity. United States.
Bedford, Ella, Tabor, Stanley, and Richardson, Charles C. Fri . "DNA polymerase with modified processivity". United States. https://www.osti.gov/servlets/purl/872606.
@article{osti_872606,
title = {DNA polymerase with modified processivity},
author = {Bedford, Ella and Tabor, Stanley and Richardson, Charles C},
abstractNote = {Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}

Works referenced in this record:

Cloning, nucleotide sequence, and expression in escherichia coli of DNA polymerase gene (polA) from thermus thermophilus HB8
journal, January 1993


A general structure for DNA-dependent DNA polymerases
journal, April 1991


Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase
journal, August 1991


Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.
journal, April 1983


Analysis of macromolecular interactions using immobilized ligands
journal, March 1992


Structure of DNA polymerase I Klenow fragment bound to duplex DNA
journal, April 1993


Biospecific interaction analysis using biosensor technology
journal, January 1993


Deoxyribonucleic Acid Synthesis in Bacteriophage SP01-infected Bacillus subtilis
journal, November 1973


Elementary steps in the DNA polymerase I reaction pathway
journal, July 1983


Escherichia coli thioredoxin stabilizes complexes of bacteriophage T7 DNA polymerase and primed templates.
journal, November 1987


Histidine Patch Thioredoxins
journal, March 1996


Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerase by in Vitro Mutagenesis
journal, April 1989