DNA polymerase with modified processivity

Bedford, Ella; Tabor, Stanley; Richardson, Charles C

Title: DNA polymerase with modified processivity

Patent · Fri Jan 01 00:00:00 EST 1999

OSTI ID:872606

Bedford, Ella ^[1]; Tabor, Stanley ^[2]; Richardson, Charles C ^[3]

Brookline, MA
Cambridge, MA
Chestnut Hill, MA

Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

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Research Organization:: Harvard University

DOE Contract Number:: FG02-88ER60688

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: US 5972603

OSTI ID:: 872606

Country of Publication:: United States

Language:: English

References (13)

Cloning, nucleotide sequence, and expression in escherichia coli of DNA polymerase gene (polA) from thermus thermophilus HB8 Asakura, Keiko; Komatsubara, Hideyuki; Soga, Shiro Journal of Fermentation and Bioengineering, Vol. 76, Issue 4 https://doi.org/10.1016/0922-338X(93)90191-A	journal	January 1993
A general structure for DNA-dependent DNA polymerases Blanco, Luis; Bernad, Antonio; Blasco, María A. Gene, Vol. 100 https://doi.org/10.1016/0378-1119(91)90346-D	journal	April 1991
Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase Myers, Thomas W.; Gelfand, David H. Biochemistry, Vol. 30, Issue 31 https://doi.org/10.1021/bi00245a001	journal	August 1991
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli. Joyce, C. M.; Grindley, N. D. Proceedings of the National Academy of Sciences, Vol. 80, Issue 7 https://doi.org/10.1073/pnas.80.7.1830	journal	April 1983
Analysis of macromolecular interactions using immobilized ligands Chaiken, Irwin; Rosé, Sergio; Karlsson, Robert Analytical Biochemistry, Vol. 201, Issue 2 https://doi.org/10.1016/0003-2697(92)90329-6	journal	March 1992
Characterization of the polA Gene of Streptococcus pneumoniae and Comparison of the DNA Polymerase I It Encodes to Homologous Enzymes from Escherichia coli and Phage T7 Lopez, P.; Martinez, S.; Diaz, A. Journal of Biological Chemistry, Vol. 264, Issue 7 https://doi.org/10.1016/S0021-9258(19)84991-8	journal	March 1989
Structure of DNA polymerase I Klenow fragment bound to duplex DNA Beese, L.; Derbyshire, V.; Steitz, T. Science, Vol. 260, Issue 5106 https://doi.org/10.1126/science.8469987	journal	April 1993
Biospecific interaction analysis using biosensor technology Malmqvist, Magnus Nature, Vol. 361, Issue 6408 https://doi.org/10.1038/361186a0	journal	January 1993
Deoxyribonucleic Acid Synthesis in Bacteriophage SP01-infected Bacillus subtilis Yehle, Clifford O.; Ganesan, A. T. Journal of Biological Chemistry, Vol. 248, Issue 21 https://doi.org/10.1016/S0021-9258(19)43312-7	journal	November 1973
Elementary steps in the DNA polymerase I reaction pathway Bryant, F. R.; Johnson, K. A.; Benkovic, S. J. Biochemistry, Vol. 22, Issue 15 https://doi.org/10.1021/bi00284a001	journal	July 1983
Escherichia coli thioredoxin stabilizes complexes of bacteriophage T7 DNA polymerase and primed templates. Huber, H. E.; Tabor, S.; Richardson, C. C. Journal of Biological Chemistry, Vol. 262, Issue 33 https://doi.org/10.1016/S0021-9258(18)47719-8	journal	November 1987
Histidine Patch Thioredoxins Lu, Zhijian; DiBlasio-Smith, Elizabeth A.; Grant, Kathleen L. Journal of Biological Chemistry, Vol. 271, Issue 9 https://doi.org/10.1074/jbc.271.9.5059	journal	March 1996
Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerase by in Vitro Mutagenesis Tabor, S.; Richardson, C. C. Journal of Biological Chemistry, Vol. 264, Issue 11 https://doi.org/10.1016/S0021-9258(18)83369-5	journal	April 1989

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Related Subjects

dna
polymerase
modified
processivity
chimeric
domain
factor
binding
naturally
associated
binding domain
dna polymerase
polymerase domain
/435/530/

Title: DNA polymerase with modified processivity

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