Convergent synthesis of proteins by kinetically controlled ligation
Abstract
The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.
- Inventors:
-
- Chicago, IL
- Boston, MA
- Issue Date:
- Research Org.:
- The University of Chicago (Chicago, IL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1014499
- Patent Number(s):
- 7674881
- Application Number:
- U S Patent Application 11/545,923
- Assignee:
- The University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- FG02-04ER63786
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Kent, Stephen, Pentelute, Brad, Bang, Duhee, Johnson, Erik, and Durek, Thomas. Convergent synthesis of proteins by kinetically controlled ligation. United States: N. p., 2010.
Web.
Kent, Stephen, Pentelute, Brad, Bang, Duhee, Johnson, Erik, & Durek, Thomas. Convergent synthesis of proteins by kinetically controlled ligation. United States.
Kent, Stephen, Pentelute, Brad, Bang, Duhee, Johnson, Erik, and Durek, Thomas. Tue .
"Convergent synthesis of proteins by kinetically controlled ligation". United States. https://www.osti.gov/servlets/purl/1014499.
@article{osti_1014499,
title = {Convergent synthesis of proteins by kinetically controlled ligation},
author = {Kent, Stephen and Pentelute, Brad and Bang, Duhee and Johnson, Erik and Durek, Thomas},
abstractNote = {The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 09 00:00:00 EST 2010},
month = {Tue Mar 09 00:00:00 EST 2010}
}
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