Title: Methods and compositions to increase the rate of ligation reactions catalyzed by a sortase

Abstract

In various embodiments methods and compositions to increase the rate and/or activity of ligation reactions catalyzed by a sortase. In certain embodiments reagents are provided that comprises, inter alia, a polypeptide comprising an amino terminal polyglycine sequence comprising at least three contiguous Gly residues (e.g., a triglycine sequence, a tetraglycine sequence, a pentaglycine sequence, etc.) followed by a peptide that is to be ligated to a moiety followed by a sequence comprising the catalytic domain of a Sortase A enzyme.

Inventors:

Clubb, Robert T.; Amer, Brendan R.

Issue Date:

Tue Sep 08 00:00:00 EDT 2020

Research Org.:

Univ. of California, Oakland, CA (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:

1735224

Patent Number(s):

10766923

Application Number:

16/072,155

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K1/13 - Labelling of peptides
C07K14/35 - from Mycobacteriaceae
C07K14/47 - from mammals
C07K2319/00 - Fusion polypeptide
C07K2319/21 - containing a His-tag
C07K2319/35 - containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
C07K2319/50 - containing protease site
C07K2319/90 - containing a motif for post-translational modification
C07K7/06 - having 5 to 11 amino acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N15/52 - Genes encoding for enzymes or proenzymes
C12N15/63 - Introduction of foreign genetic material using vectors
C12N2310/3181 - Peptide nucleic acid, PNA
C12N2310/3517 - Marker
C12N9/52 - derived from bacteria {or Archaea}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y304/2207 - Sortase A

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DOE Contract Number:  

FC02-02ER63421

Resource Type:

Patent

Resource Relation:

Patent File Date: 01/26/2017

Country of Publication:

United States

Language:

English

Subject:

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY