Repressor-mediated tissue-specific gene expression in plants

Meagher, Richard B; Balish, Rebecca S; Tehryung, Kim; McKinney, Elizabeth C

Title: Repressor-mediated tissue-specific gene expression in plants

Patent · Tue Feb 17 00:00:00 EST 2009

OSTI ID:988296

Meagher, Richard B ^[1]; Balish, Rebecca S ^[2]; Tehryung, Kim ^[1]; McKinney, Elizabeth C ^[1]

Athens, GA
Oxford, OH

Plant tissue specific gene expression by way of repressor-operator complexes, has enabled outcomes including, without limitation, male sterility and engineered plants having root-specific gene expression of relevant proteins to clean environmental pollutants from soil and water. A mercury hyperaccumulation strategy requires that mercuric ion reductase coding sequence is strongly expressed. The actin promoter vector, A2pot, engineered to contain bacterial lac operator sequences, directed strong expression in all plant vegetative organs and tissues. In contrast, the expression from the A2pot construct was restricted primarily to root tissues when a modified bacterial repressor (LacIn) was coexpressed from the light-regulated rubisco small subunit promoter in above-ground tissues. Also provided are analogous repressor operator complexes for selective expression in other plant tissues, for example, to produce male sterile plants.

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Research Organization:: University of Georgia Research Foundation, Inc. (Athens, GA)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG07-02ER63493

Assignee:: University of Georgia Research Foundation, Inc. (Athens, GA)

Patent Number(s):: 7,491,811

Application Number:: 11/038,900

OSTI ID:: 988296

Country of Publication:: United States

Language:: English

References (58)

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