Amplification of trace amounts of nucleic acids
Patent
·
OSTI ID:983051
- Brookline, MA
- Brighton, MA
Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. "Primer-dimer" background can be reduced by judicious design of primers. We have shown clean signal-to-noise with as little as starting material as one single human cell (.about.6 picogram), E. coli cell (.about.5 femtogram) or Prochlorococcus cell (.about.3 femtogram).
- Research Organization:
- Harvard University
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-02ER63445
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Number(s):
- 7,387,876
- Application Number:
- 11/066,559
- OSTI ID:
- 983051
- Country of Publication:
- United States
- Language:
- English
Whole-genome amplification of DNA from residual cells left by incidental contact
|
journal | January 2004 |
Comprehensive human genome amplification using multiple displacement amplification
|
journal | April 2002 |
Similar Records
Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography
Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using
Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using
Journal Article
·
Sun Apr 22 00:00:00 EDT 2007
· Nucleic Acids Research
·
OSTI ID:983051
Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using
Patent
·
Tue Jun 27 00:00:00 EDT 1995
·
OSTI ID:983051
Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using
Patent
·
Sun Jan 01 00:00:00 EST 1995
·
OSTI ID:983051