Evaluation of the contribution of the renal capsule and cortex to kidney autofluorescence intensity under ultraviolet excitation
The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not to date been addressed. In this work the relative contribution to rat kidney autofluorescence of the capsule vs. cortex under ultraviolet excitation is determined from experimental results obtained using autofluorescence microscopy and a suitable mathematical model. The results allow for a quantitative assessment of the relative contribution of the signal originating in the metabolically active cortex as a function of capsule thickness for different wavelengths.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 952764
- Report Number(s):
- LLNL-JRNL-409415; TRN: US200914%%97
- Journal Information:
- Journal of Biomedical Optics, vol. 14, no. 2, April 30, 2009, pp. 020505-1 to 020505-3, Vol. 14, Issue 2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Single sample extraction and HPLC processing for quantification of NAD and NADH levels in Saccharomyces cerevisiae
Autofluorescence dynamics during reperfusion following long-term renal ischemia in a rat model