Field-Portable Immunoassay Instruments and Reagents to Measure Chelators and Mobile Forms of Uranium
The goals for the 3-year project period are (1) to test and validate the present uranium sensor and develop protocols for its use at the NABIR Field Research Center; (2) to develop new reagents that will provide superior performance for the present hand-held immunosensor; and (3) to develop new antibodies that will permit this sensor to also measure other environmental contaminants (chromium, mercury, and/or DTPA). Sensor design modifications are underway via international collaborations. New reagents that will provide superior performance for the present hand-held immunosensor are being prepared and tested. New methods have been developed, to produce recombinant forms of metal-specific monoclonal antibodies for use with the sensor. Site-directed mutagenesis experiments are underway to determine the mechanisms of binding. Immunization experiments with sheep and rabbits to develop new recombinant forms of antibodies to metal-chelate complexes (chromium, mercury, and/or DTPA) have been initiated.
- Research Organization:
- Tulane University, New Orleans, LA
- Sponsoring Organization:
- USDOE Office of Science (SC)
- OSTI ID:
- 893777
- Report Number(s):
- NABIR-1011983-2003; R&D Project: NABIR 1011983; TRN: US0606109
- Country of Publication:
- United States
- Language:
- English
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