Imaging of Tissue Micro-Structures using a Multi-Modal Microscope Design

Journal Article · Fri Aug 12 00:00:00 EDT 2005 · Journal of Selected Topics in Quantum Electronics

OSTI ID:883599

Demos, S G; Lieber, C A; Lin, B; Ramsamooj, R

We investigate a microscope design that offers high signal sensitivity and hyperspectral imaging capabilities and allows for implementation of various optical imaging approaches while its operational complexity is minimized. This system utilizes long working distance microscope objectives that enable for off-axis illumination of the tissue thereby allowing for excitation at any optical wavelength and nearly eliminating spectral noise from the optical elements. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissue microstructures using autofluorescence and light scattering imaging methods.

• Research Organization: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 883599
• Report Number(s): UCRL-JRNL-214641; TRN: US200615%%166
• Journal Information: Journal of Selected Topics in Quantum Electronics, Vol. 11, Issue 4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
DESIGN
EXCITATION
ILLUMINANCE
IMPLEMENTATION
LIGHT SCATTERING
MICROSCOPES
SENSITIVITY
WAVELENGTHS