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Title: Multiflavor streptavidin

Patent ·
OSTI ID:874334
 [1];  [2];  [3];  [1];  [4]
  1. Boston, MA
  2. Needham, MA
  3. Medfield, MA
  4. Del Mar, CA
+ Show Author Affiliations

Compounds and methods are described for producing streptavidin mutants with changed affinities. In particular, modifications to the sequence of the natural streptavidin gene is described to create amino acid substitutions resulting in greater affinity for biotin substitutes than for biotin.

Research Organization:
Boston University
DOE Contract Number:
FG02-93ER61656
Assignee:
The Trustees of Boston University (Boston, MA)
Patent Number(s):
US 6368813
OSTI ID:
874334
Country of Publication:
United States
Language:
English

References (4)

Influence of endogenous biotin on the biodistribution of labelled biotin derivatives in mice journal January 1995
Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120. journal February 1995
Molecular Origins of the Slow Streptavidin-Biotin Dissociation Kinetics journal November 1995
Intermolecular forces and energies between ligands and receptors journal October 1994

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+3 more

Related Subjects

multiflavor
streptavidin
compounds
methods
described
producing
mutants
changed
affinities
modifications
sequence
natural
create
amino
acid
substitutions
resulting
affinity
biotin
substitutes
amino acid
/435/530/999/