Encapsulation of biomaterials in porous glass-like matrices prepared via an aqueous colloidal sol-gel process
- Richmond, CA
- Swarthmore, PA
The present invention provides a process for the encapsulation of biologically important proteins into transparent, porous silica matrices by an alcohol-free, aqueous, colloidal sol-gel process, and to the biological materials encapsulated thereby. The process is exemplified by studies involving encapsulated cytochrome c, catalase, myoglobin, and hemoglobin, although non-proteinaceous biomaterials, such as active DNA or RNA fragments, cells or even tissues, may also be encapsulated in accordance with the present methods. Conformation, and hence activity of the biomaterial, is successfully retained after encapsulation as demonstrated by optical characterization of the molecules, even after long-term storage. The retained conformation of the biomaterial is strongly correlated to both the rate of gelation and the subsequent drying speed of the encapsulatng matrix. Moreover, in accordance with this process, gelation is accelerated by the use of a higher colloidal solid concentration and a lower synthesis pH than conventional methods, thereby enhancing structural stability and retained conformation of the biomaterials. Thus, the invention also provides a remarkable improvement in retaining the biological activity of the encapsulated biomaterial, as compared with those involved in conventional alkoxide-based processes. It further provides new methods for the quantitative and qualitative detection of test substances that are reactive to, or catalyzed by, the active, encapsulated biological materials.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia, PA (United States)
- DOE Contract Number:
- FG02-97ER45637
- Assignee:
- The Trustees of the University of Pennsylvania
- Patent Number(s):
- US 6303290
- OSTI ID:
- 874055
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
biomaterials
porous
glass-like
matrices
prepared
via
aqueous
colloidal
sol-gel
process
provides
biologically
proteins
transparent
silica
alcohol-free
biological
materials
encapsulated
exemplified
studies
involving
cytochrome
catalase
myoglobin
hemoglobin
non-proteinaceous
active
dna
rna
fragments
cells
tissues
methods
conformation
hence
activity
biomaterial
successfully
retained
demonstrated
optical
characterization
molecules
long-term
storage
strongly
correlated
rate
gelation
subsequent
drying
speed
encapsulatng
matrix
moreover
accelerated
solid
concentration
synthesis
ph
conventional
enhancing
structural
stability
remarkable
improvement
retaining
compared
involved
alkoxide-based
processes
quantitative
qualitative
detection
substances
reactive
catalyzed
conventional methods
long-term storage
sol-gel process
porous silica
porous glass
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