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Title: Method for facilitating the introduction of material into cells

Patent ·
OSTI ID:872991

The present invention is a method for creating a localized disruption within a boundary of a cell or structure by exposing a boundary of a cell or structure to a set of energetically charged particles while regulating the energy of the charged particles so that the charged particles have an amount of kinetic energy sufficient to create a localized disruption within an area of the boundary of the cell or structure, then upon creation of the localized disruption, the amount of kinetic energy decreases to an amount insufficient to create further damage within the cell or structure beyond the boundary. The present invention is also a method for facilitating the introduction of a material into a cell or structure using the same methodology then further exciting the area of the boundary of the cell or structure where the localized disruption was created so to create a localized temporary opening within the boundary then further introducing the material through the temporary opening into the cell or structure.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
DOE Contract Number:
AC05-96OR22464
Assignee:
Lockheed Martin Energy Research Corporation (Oak Ridge, TN)
Patent Number(s):
US 6060315
OSTI ID:
872991
Country of Publication:
United States
Language:
English

References (4)

DNA-Mediated Gene Transfer Efficiency Is Enhanced by Ionizing and Ultraviolet Irradiation of Rodent Cells in Vitro: I. Kinetics of Enhancement journal November 1985
Physiology of Crop Production book January 1995
Kinetics of electroinduced pores as a probe of membrane modification produced by ionizing radiation journal May 1997
Overcoming the Obstacles to Gene Therapy journal June 1997