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Title: Apparatus for reducing solvent luminescence background emissions

Patent ·
OSTI ID:871965
 [1];  [1];  [2];  [3];  [4];  [1];  [1];  [5];  [1]
  1. Los Alamos, NM
  2. Charlottesville, VA
  3. Jemez Springs, NM
  4. Pittsburgh, PA
  5. Sante Fe, NM
+ Show Author Affiliations

The detectability of luminescent molecules in solution is enhanced by reducing the background luminescence due to impurity species also present in the solution. A light source that illuminates the solution acts to photolyze the impurities so that the impurities do not luminesce in the fluorescence band of the molecule of interest. Molecules of interest may be carried through the photolysis region in the solution or may be introduced into the solution after the photolysis region.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
DOE Contract Number:
W-7405-ENG-36
Assignee:
Regents of University of California (Los Alamos, NM)
Patent Number(s):
US 5834204
OSTI ID:
871965
Country of Publication:
United States
Language:
English

References (7)

Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence
  • Goodwin, Peter M.; Wilkerson, Jr., Charles W.; Ambrose, W. Patrick
  • OE/LASE'93: Optics, Electro-Optics, & Laser Applications in Science& Engineering, SPIE Proceedings https://doi.org/10.1117/12.146731
conference June 1993
Detection of single fluorescent molecules journal November 1990
DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA
  • Goodwin, Peter M.; Schecker, Jay A.; Wilkerson, Jr., Charles W.
  • OE/LASE'93: Optics, Electro-Optics, & Laser Applications in Science& Engineering, SPIE Proceedings https://doi.org/10.1117/12.146712
conference June 1993
The Photophysical Constants of Several Fluorescent dyes Pertaining to Ultrasensitive Fluorescence Spectroscopy journal May 1993
Photostability studies of phycobiliprotein fluorescent labels journal March 1987
Single molecule detection and photochemistry on a surface using near-field optical excitation journal January 1994
Laser-Induced Fluorescence Detection of Rhodamine-6G at 6 × 10 −15 M journal June 1991

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Related Subjects

apparatus
reducing
solvent
luminescence
background
emissions
detectability
luminescent
molecules
solution
enhanced
due
impurity
species
light
source
illuminates
photolyze
impurities
luminesce
fluorescence
band
molecule
carried
photolysis
region
introduced
photolysis region
background emissions
light source
reducing solvent
solvent luminescence
impurity species
luminescence background
luminescence due
luminescent molecules
background luminescence
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