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Title: Site directed recombination

Patent ·
OSTI ID:871268
 [1]
  1. Los Altos, CA
+ Show Author Affiliations

Enhanced homologous recombination is obtained by employing a consensus sequence which has been found to be associated with integration of repeat sequences, such as Alu and ID. The consensus sequence or sequence having a single transition mutation determines one site of a double break which allows for high efficiency of integration at the site. By introducing single or double stranded DNA having the consensus sequence flanking region joined to a sequence of interest, one can reproducibly direct integration of the sequence of interest at one or a limited number of sites. In this way, specific sites can be identified and homologous recombination achieved at the site by employing a second flanking sequence associated with a sequence proximal to the 3'-nick.

Research Organization:
Genetic Information Research Institute (Palo Alto, CA)
DOE Contract Number:
FG03-95ER62139
Assignee:
Genetic Information Research Institute (Palo Alto, CA)
Patent Number(s):
US 5695977
OSTI ID:
871268
Country of Publication:
United States
Language:
English

References (7)

Integration site preferences of the Alu family and similar repetitive DNA sequences journal January 1985
Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells. journal June 1994
Structure of two human beta-actin-related processed genes one of which is located next to a simple repetitive sequence. journal May 1983
Nonviral Retroposons: Genes, Pseudogenes, and Transposable Elements Generated by the Reverse Flow of Genetic Information journal June 1986
Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes. journal January 1995
Direct repeats flank three small nuclear RNA pseudogenes in the human genome journal October 1981
mRNA retroposition in human cells: processed pseudogene formation. journal December 1995

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site
directed
recombination
enhanced
homologous
obtained
employing
consensus
sequence
found
associated
integration
repeat
sequences
alu
single
transition
mutation
determines
double
break
allows
efficiency
introducing
stranded
dna
flanking
region
joined
reproducibly
direct
limited
sites
specific
identified
achieved
proximal
-nick
stranded dna
double stranded
repeat sequences
repeat sequence
consensus sequence
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