A Nucleocytoplasmic Shuttling Protein in Oxidative Stress Tolerance
Plants for effective extraction of toxic metals and radionuclides must tolerate oxidative stress. To identify genes that enhance oxidative stress tolerance, an S. pombe cDNA expression plasmid library was screened for the ability to yield hypertolerant colonies. Here, we report on the properties of one gene that confers hypertolerance to cadmium and oxidizing chemicals. This gene appears to be conserved in other organisms as homologous genes are found in human, mouse, fruitfly and Arabidopsis. The fruitfly and Arabidopsis genes likewise enhance oxidative stress tolerance in fission yeast. During oxidative stress, the amount of mRNA does not change, but protein fusions to GFP relocate from the cytoplasm to the nucleus. The same pattern is observed with the Arabidopsis homologue-GFP fusion protein. This behavior suggests a signaling role in oxidative stress tolerance and these conserved proteins may be targets for engineering stress tolerant plants for phytoremediation.
- Research Organization:
- U.S. Department of Agriculture, Albany, CA; University of California, Berkeley, CA (US)
- Sponsoring Organization:
- USDOE Office of Science (SC) (US)
- OSTI ID:
- 826383
- Resource Relation:
- Conference: 225th American Chemical Society Meeting, New Orleans, LA (US), 03/23/2003--03/27/2003; Other Information: PBD: 26 Mar 2003
- Country of Publication:
- United States
- Language:
- English
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