Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts
This project focuses on lesions in the plasma membrane of protoplasts that occur during freezing to temperatures below {minus}5{degrees} which result in changes in the semipermeablity of the plasma membrane. This injury, referred to as loss of osmotic responsiveness, is associated with the formation of large, aparticulate domains in the plasma membrane, aparticulate lamellae subtending the plasma membrane, and lamellar-to-hexagonal{sub II} phase transitions in the plasma membrane and subtending lamellar. The goals of this project are to provide a mechanistic understanding of the mechanism by which freeze-induced dehydration effects the formation of aparticulate domains and lamellar-to-hexagonal{sub II} phase transitions and to determine the mechanisms by which cold acclimation and cryoprotectants preclude or diminish these ultrastructural changes. Our working hypothesis is the formation of aparticulate domains and lamellar-to-hexagon{sub II} phase transitions in the plasma membrane and subtending lamellae are manifestations of hydration-dependent bilayer-bilayer interactions.
- Research Organization:
- Cornell Univ., Ithaca, NY (United States). Dept. of Soil, Crop and Atmospheric Sciences
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-84ER13214
- OSTI ID:
- 7302592
- Report Number(s):
- DOE/ER/13214-7; ON: DE92018399
- Country of Publication:
- United States
- Language:
- English
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Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts, progress report
Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts. Progress report, May 16, 1992--January 9, 1993
Related Subjects
09 BIOMASS FUELS
60 APPLIED LIFE SCIENCES
CELL MEMBRANES
CONFORMATIONAL CHANGES
FREEZING
BIOLOGICAL EFFECTS
BIOLOGICAL ADAPTATION
CRYOBIOLOGY
GLYCOLS
LAMELLAE
LEAVES
LIPIDS
OSMOSIS
PLANT CELLS
PROGRESS REPORT
RYE
SORBITOL
ALCOHOLS
BIOLOGY
CARBOHYDRATES
CELL CONSTITUENTS
CEREALS
DIFFUSION
DIURETICS
DOCUMENT TYPES
DRUGS
GRAMINEAE
HYDROXY COMPOUNDS
LILIOPSIDA
MAGNOLIOPHYTA
MEMBRANES
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
560400* - Other Environmental Pollutant Effects
090800 - Biomass Fuels- Production- (1990-)
553000 - Agriculture & Food Technology