Human Chromosome 21: Mapping of the chromosomes and cloning of cDNAs
The objective of the research funded by DOE grant DE-FG02-89ER60857 from 6/15/89 to 8/31/91 was to contribute to the physical mapping of human chromosome 21 (HC21) by cloning large fragments of DNA into Yeast Artificial Chromosomes (YACs) and identify YACs that map on HC21. A total of 54 sequence tagged sites (STS) have been developed and mapped in our laboratory to HC21 and can be used as initial reference points for YAC identification and construction of overlapping clones. A small YAC library was constructed which is HC21 specific. DNA from somatic cell hybrid WAV17 or from flow-sorted HC21 was partially digested with EcoRI, ligated into vectors PJS97, PJS98, and YACs have been obtained with average size insert of more than 300 kb. This library has been deposited in D. Patterson's lab for the Joint YAC screening effort. Additional YAC libraries from ICI Pharmaceuticals or from Los Alamos National Laboratories have been screened with several STS and positive YACs have been identified. Work in progress includes screening of YAC libraries in order to construct overlapping clones, characterization of the cloning ends of YACs, characterization of additional STS and cloning of HC21 specific cDNAs. 15 refs., 2 figs., 5 tabs.
- Research Organization:
- Johns Hopkins Univ., Baltimore, MD (United States). School of Medicine
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-89ER60857
- OSTI ID:
- 6397375
- Report Number(s):
- DOE/ER/60857-3; ON: DE92003412
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HUMAN CHROMOSOME 21
DNA SEQUENCING
DNA-CLONING
GENETIC MAPPING
BIOCHEMISTRY
CHROMOSOME SORTING
DATA ANALYSIS
ELECTROPHORESIS
GENETICS
OLIGONUCLEOTIDES
PLASMIDS
PROBES
PROGRESS REPORT
BIOLOGY
CELL CONSTITUENTS
CHEMISTRY
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DOCUMENT TYPES
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
550200 - Biochemistry