Mutagenicity of nitrogen compounds from synthetic crude oils: collection, separation and biological testing
In order to determine the long range health effects such as carcinogenicity/mutagenicity/teratogenicity/toxicity, associated with the newly emerging energy technologies, we have utilized the Ames Salmonella assay to evaluate mutagenic properties of synthetic fuels. Coupling with class fractionation was necessary. Organic extraction and liquid/liquid partitioning was used to separate acidic and basic fraction. The neutral material was separated using Sephadex LH-20 gel filtration into saturated and aromatic fractions of various ring sizes. The alkaline fraction was subfractionated eluting with benzene and ethanol on a basic alumina column and then with isopropanol and acetone using a Sephadex LH-20 gel column. The frameshift strain TA-98 was utilized along with Aroclor-induced rat liver homogenate (S-9 mix) for the mutagenicity assay. The natural crude oils were slightly mutagenic, the polynucleararomatics constituting the activity, while the coal-derived fuels indicated mutagenicity associated with alkaline constituents as well as polyaromatics. Hydrotreated coal (H-coal, HDT) or Shale (Paraho-Shale oil, HDT) derived fuels were not mutagenic. Ninety percent of the mutagenic activity in alkaline fraction was recovered in the acetone subfraction. High resolution spectroscopy of this fraction indicates polycyclic aromatic primary amines along with azaarenes as organic constituents responsible for the mutagenic activity associated with shale- and coal-derived fuels.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5704769
- Report Number(s):
- CONF-800323-1
- Resource Relation:
- Conference: Symposium on the application of short-term bioassays in the fractionation and analysis of complex environmental mixtures, Williamsburg, VA, USA, 4 Mar 1980
- Country of Publication:
- United States
- Language:
- English
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01 COAL, LIGNITE, AND PEAT
04 OIL SHALES AND TAR SANDS
COAL LIQUIDS
MUTAGEN SCREENING
POLYCYCLIC AROMATIC HYDROCARBONS
SHALE OIL FRACTIONS
SYNTHETIC PETROLEUM
CARCINOGENESIS
MUTAGENESIS
TERATOGENESIS
TOXICITY
EXPERIMENTAL DATA
FRACTIONATION
H-COAL PROCESS
HYDROGENATION
ISOLATED VALUES
MUTATIONS
PARAHO PROCESS
SALMONELLA
AROMATICS
BACTERIA
CHEMICAL REACTIONS
COAL LIQUEFACTION
DATA
DATA FORMS
ENERGY SOURCES
FOSSIL FUELS
FUELS
HYDROCARBONS
INFORMATION
LIQUEFACTION
MICROORGANISMS
MINERAL OILS
NUMERICAL DATA
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PATHOGENESIS
SCREENING
SEPARATION PROCESSES
SHALE OIL
SYNTHETIC FUELS
THERMOCHEMICAL PROCESSES
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