Genetic changes in Mammalian cells transformed by helium cells
- Naples Univ. (Italy). Dipt. di Scienze Fisiche
- Lawrence Berkeley Lab., CA (USA)
Midterm Syrian Hamster embryo (SHE) cells were employed to study high LET-radiation induced tumorigenesis. Normal SHE cells (secondary passage) were irradiated with accelerated helium ions at an incident energy of 22 MeV/u (9--10 keV/{mu}m). Transformed clones were isolated after growth in soft agar of cells obtained from the foci of the initial monolayer plated postirradiation. To study the progression process of malignant transformation, the transformed clones were followed by monolayer subculturing for prolonged periods of time. Subsequently, neoplasia tests in nude mice were done. In this work, however, we have focused on karyotypic changes in the banding patterns of the chromosomes during the early part of the progressive process of cell transformation for helium ion-induced transformed cells. 26 refs., 5 figs., 2 tabs.
- Research Organization:
- Lawrence Berkeley Lab., CA (USA)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 5639541
- Report Number(s):
- LBL-30025; CONF-9006220-10; ON: DE91013778
- Resource Relation:
- Conference: COSPAR plenary conference, The Hague (Netherlands), 25 Jun - 6 Jul 1990
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
EMBRYONIC CELLS
ONCOGENIC TRANSFORMATIONS
BANDING TECHNIQUES
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL PROLIFERATION
COLONY FORMATION
GENOME MUTATIONS
HAMSTERS
HELIUM IONS
ION BEAMS
KARYOTYPE
LET
MORPHOLOGICAL CHANGES
RBE
STRAND BREAKS
ANIMAL CELLS
ANIMALS
BEAMS
BIOLOGICAL EFFECTS
CELL TRANSFORMATIONS
CHARGED PARTICLES
CYTOLOGICAL TECHNIQUES
ENERGY TRANSFER
IONS
MAMMALS
MUTATIONS
RADIATION EFFECTS
RODENTS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture