(A structural assessment of the role of the cell surface carbohydrates of Rhizobium in the Rhizobium/legume symbiosis)

Technical Report · Tue Jan 01 00:00:00 EST 1991

DOI:https://doi.org/10.2172/5242472· OSTI ID:5242472

Hollingsworth, R I

Research continued on the study of cell surface carbohydrates of Rhizobium. Objectives include: To characterize, at a structural level, the differences between the lipopolysaccharides of a representative number of strains from different Rhizobium species to determine which features of LPS structure are species-specific and might, therefore, be determinants of host specificity. Determine the effect(s) of nod gene induction on the structure of Rhizobium lipopolysaccharides and determine whether synthesis of a modified LPS molecule or a new surface glycoconjugate is initiated by nod gene induction. Develop a non-chemical means for rapidly screening large numbers of bacterial strains in order to determine which glycoconjugate structural features are conserved between strains of the same species. Provide the necessary structural information which, when coupled with developments in the rapidly expanding field of Rhizobium genetics, should lead to a clear understanding of the role of Rhizobium surface glycoconjugates in host/symbiont interactions. Progress is discussed.

• Research Organization: Michigan State Univ., East Lansing, MI (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-89ER14029
• OSTI ID: 5242472
• Report Number(s): DOE/ER/14029-2; ON: DE92014590
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIPOPOLYSACCHARIDES
MOLECULAR STRUCTURE
GENES
PLANTS
PROGRESS REPORT
RHIZOBIUM
SPECIFICITY
STRUCTURE-ACTIVITY RELATIONSHIPS
SYMBIOSIS
BACTERIA
CARBOHYDRATES
DOCUMENT TYPES
LIPIDS
MICROORGANISMS
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
550200* - Biochemistry
550700 - Microbiology
550400 - Genetics